鸡重组脂联素增强油酸和棕榈酸处理的LMH细胞的脂肪酸代谢

IF 1 4区农林科学 Q3 Agricultural and Biological Sciences

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2022-01-01 DOI:10.37496/rbz5120220087

Yi-Ru Zhuang, Yuan-Yu Lin

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引用次数: 1

摘要

本研究的目的是探讨重组脂联素对鸡肝细胞的影响。通过PCR扩增鸡脂联素全长基因，将其克隆到载体pET-32a中，转化至大肠杆菌BL21。利用SDS-PAGE对表达的重组蛋白进行纯度检测和分析。用1 mM IPTG在30℃下诱导3 h，用Ni-NTA亲和层析纯化重组硫氧还毒素-脂联素融合蛋白。成功地在细菌系统中表达和纯化了鸡脂联素。此外，鸡重组脂联素表明，它改善了棕榈酸和油酸诱导的脂肪生成，其中可能涉及β-氧化的增加和脂肪生成相关基因的减少。综上所述，鸡重组脂联素增强了LMH细胞的脂肪酸代谢。

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Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells

- The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.

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来源期刊

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science 农林科学-奶制品与动物科学

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： The Revista Brasileira de Zootecnia (RBZ; Brazilian Journal of Animal Science) encompasses all fields of Animal Science Research. The RBZ publishes original scientific articles in the areas of Aquaculture, Biometeorology and Animal Welfare, Forage Crops and Grasslands, Animal and Forage Plants Breeding and Genetics, Animal Reproduction, Ruminant and Non-Ruminant Nutrition, and Animal Production Systems and Agribusiness.