miR-34a在膀胱癌中的表达及作用

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2012-12-15 DOI:10.3760/CMA.J.ISSN.1000-6702.2012.12.017

Wei Wang, Tan Li, G. Han, Ying Li, Li-hua Shi, Hui Li

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引用次数: 16

摘要

为了研究microRNA-34a (miR-34a)在膀胱癌中的生物学功能，我们采用实时荧光定量聚合酶链反应(qRT-PCR)检测了42例膀胱癌患者miR-34a的表达。我们还研究了miR-34a表达与膀胱癌发生发展的关系。化学合成成熟的miR-34a模拟物并将其瞬时转染到人膀胱癌T24细胞中。通过流式细胞术和MTT分别评价miR-34a对T24细胞凋亡、细胞周期和增殖的影响。结果显示，miR-34a的低表达率与膀胱癌的恶性程度及肿瘤大小相关。T24细胞中miR-34a的上调有助于细胞生长和细胞周期阻滞，但不参与caspase-3途径。这些发现提示miRNA-34a的相对低表达可能参与了膀胱癌的发生。

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Expression and role of miR-34a in bladder cancer.

To investigate the biological function of microRNA-34a (miR-34a) in bladder cancer, the expression of miR-34a was determined using quantitative real-time polymerase chain (qRT-PCR) reaction in 42 cases of bladder cancer. The relationship between the expression of miR-34a and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and transiently transfected into human bladder cancer T24 cells. The effects of miR-34a on apoptosis, cell cycle and proliferation in T24 cells were evaluated by flow cytometry and MTT, respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size of bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell cycle arrest, but not caspase-3 pathway. These findings suggest that the relative low expression of miRNA-34a might be involved in the tumorigenesis of bladder cancer.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.