一种新的产腈酶Pantoea sp.及其酶学性质:一种新的产腈酶Pantoea sp.及其酶学性质

Q4 Agricultural and Biological Sciences

应用与环境生物学报 Pub Date : 2013-12-19 DOI:10.3724/SP.J.1145.2013.00346

Mingle Cao, Xinglin Jiang, Haibo Zhang, M. Xian, Xin Xu, Wei Liu

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引用次数: 0

摘要

腈酶能在温和条件下高效水解腈。腈酶法具有低污染、低成本等优点，在农业、工业、生物医药和环境保护等领域有着广泛的应用。市场上缺乏具有广泛底物特异性的腈酶。本研究采用Berthelot法和高效液相色谱法分离鉴定了一株能水解乙腈、4-羟基苯基氰化物和3-羟基丙腈的新腈酶Pantoea sp. 1-2。以硫酸铵为氮源，产氮量为16.45 U/mL。在pH 6.6 ~ 7.6、温度22 ~ 45℃条件下，酶活性保持80%，孵育30 h后保持稳定。该菌株具有重要的工业利用价值。图7，文献21

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A New Nitrilase Producing Pantoea sp. and Its Enzymological Properties: A New Nitrilase Producing Pantoea sp. and Its Enzymological Properties

Nitrilases can hydrolyze nitrile efficiently under mild conditions. For their low pollution and cost, the enzymatic methods with nitrilases are widely used in agriculture, industry, biomedicine and environment protection. Nitrilases with broad substrate specificity are lacked in the nitrilase market. In this study, Berthelot method and high performance liquid chromatography were used to separate and identify a new strain of nitrilase Pantoea sp. 1-2, which can hydrolyze acetonitrile, 4-hydroxyphenylcyanide and 3-hydroxypropionitrile. The strain produced 16.45 U/mL nitrilase with ammonium sulfate as nitrogen source. The enzyme remained 80% activity at pH 6.6-7.6, temperature 22-45 °C, staying stable after 30 h incubation. The strain would be of great value in industrial utilization. Fig 7, Ref 21

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来源期刊

应用与环境生物学报 Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

4458

期刊介绍： The “Chinese Journal of Applied and Environmental Biology” is a national scientific and technological journal published both at home and abroad. It was started in 1995 and became bimonthly in 1999, and now it is a core journal for applied and environmental biology in China. It aims to introduce and exchange the new achievements, new technologies, new methods and new progresses made from the research fields of biology and its related disciplines both in China and abroad; enhance scientific and technological research serving for the development and construction of national economy, and improvement of people’s living standards.