Jie Liu, Die Hu, Hai-jia Chu, Juan Yan, Jian-qiang Li
{"title":"紫花苜蓿抗旱、耐盐EST-SSR标记的筛选。","authors":"Jie Liu, Die Hu, Hai-jia Chu, Juan Yan, Jian-qiang Li","doi":"10.3724/SP.J.1142.2013.50493","DOIUrl":null,"url":null,"abstract":"To provide genetic markers for the study of ecological adaptability to responsive drought ̄ and salinity ̄stress for Medicago ruthenica Trautv.ꎬ we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought ̄and salinity ̄responsive EST ̄ SSR markers published for chickpea. DNA sequences were amplified with eight pairs of SSR primers by SSR ̄PCR technique. One hundred and eleven alleles were detectedꎬ on averageꎬ 13 88 alleles were detected per site. The mean values of observed heterozygosity and expected heterozygosity were 0 497 and 0 687ꎬ respectively. Polymorphism information content value ranged from 0 313 to 0 883ꎬ and the mean value was 0 649. These results indicate that the eight EST ̄SSR markers can be used to estimate genetic diversity of M. ruthenicaꎬ and genetic diversity was at a high level. Polymorphism EST ̄SSR markers can be used to investigate ecological adaptive evolution. The results contribute to revealing the genetic variation of drought ̄ and salt ̄tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M. ruthenica populations.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"493"},"PeriodicalIF":0.0000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Screening of Drought- and Salinity-responsive EST-SSR Markers inMedicago ruthenicaTrautv.\",\"authors\":\"Jie Liu, Die Hu, Hai-jia Chu, Juan Yan, Jian-qiang Li\",\"doi\":\"10.3724/SP.J.1142.2013.50493\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To provide genetic markers for the study of ecological adaptability to responsive drought ̄ and salinity ̄stress for Medicago ruthenica Trautv.ꎬ we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought ̄and salinity ̄responsive EST ̄ SSR markers published for chickpea. DNA sequences were amplified with eight pairs of SSR primers by SSR ̄PCR technique. One hundred and eleven alleles were detectedꎬ on averageꎬ 13 88 alleles were detected per site. The mean values of observed heterozygosity and expected heterozygosity were 0 497 and 0 687ꎬ respectively. Polymorphism information content value ranged from 0 313 to 0 883ꎬ and the mean value was 0 649. These results indicate that the eight EST ̄SSR markers can be used to estimate genetic diversity of M. ruthenicaꎬ and genetic diversity was at a high level. Polymorphism EST ̄SSR markers can be used to investigate ecological adaptive evolution. The results contribute to revealing the genetic variation of drought ̄ and salt ̄tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M. ruthenica populations.\",\"PeriodicalId\":20134,\"journal\":{\"name\":\"Plant Science Journal\",\"volume\":\"31 1\",\"pages\":\"493\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Science Journal\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"https://doi.org/10.3724/SP.J.1142.2013.50493\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Journal","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.3724/SP.J.1142.2013.50493","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Screening of Drought- and Salinity-responsive EST-SSR Markers inMedicago ruthenicaTrautv.
To provide genetic markers for the study of ecological adaptability to responsive drought ̄ and salinity ̄stress for Medicago ruthenica Trautv.ꎬ we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought ̄and salinity ̄responsive EST ̄ SSR markers published for chickpea. DNA sequences were amplified with eight pairs of SSR primers by SSR ̄PCR technique. One hundred and eleven alleles were detectedꎬ on averageꎬ 13 88 alleles were detected per site. The mean values of observed heterozygosity and expected heterozygosity were 0 497 and 0 687ꎬ respectively. Polymorphism information content value ranged from 0 313 to 0 883ꎬ and the mean value was 0 649. These results indicate that the eight EST ̄SSR markers can be used to estimate genetic diversity of M. ruthenicaꎬ and genetic diversity was at a high level. Polymorphism EST ̄SSR markers can be used to investigate ecological adaptive evolution. The results contribute to revealing the genetic variation of drought ̄ and salt ̄tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M. ruthenica populations.
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