乌干达非正规生牛奶中布鲁氏菌种类的分子检测和特性分析

Q1 Environmental Science

Infection Ecology and Epidemiology Pub Date : 2016-01-01 DOI:10.3402/iee.v6.32442

T. Hoffman, K. Rock, D. Mugizi, S. Muradrasoli, Elisabeth Lindahl-Rajala, J. Erume, U. Magnusson, Å. Lundkvist, S. Boqvist

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引用次数: 24

摘要

本研究在乌干达的非正式牛奶链中鉴定并鉴定了布鲁氏菌的种类。采用实时荧光定量PCR技术，以bcsp31基因为引物，对324份牛散装乳样品进行布鲁氏菌鉴定。在检测的样本中，6.5%的布鲁氏菌呈阳性。在comp25的系统发育中，研究序列被发现在含有B. abortus序列的分支中形成一个单独的分支。该研究表明，乌干达非正规销售的牛乳可能是人类布鲁氏菌病的一个危险因素，并证实牛群中存在流产杆菌。这一信息对今后可能采取的控制措施(如牛的疫苗接种)很重要。

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Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda

This study identified and characterized Brucella species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus Brucella by real-time PCR with primers targeting the bcsp31 gene and further characterized by the omp25 gene. Of the samples tested, 6.5% were positive for Brucella species. In the omp25 phylogeny, the study sequences were found to form a separate clade within the branch containing B. abortus sequences. The study shows that informally marketed cattle milk in Uganda is a likely risk factor for human brucellosis and confirms that B. abortus is present in the cattle population. This information is important for potential future control measures, such as vaccination of cattle.

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来源期刊

Infection Ecology and Epidemiology Environmental Science-Environmental Science (miscellaneous)

CiteScore

8.70

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment