脱落酸诱导的25 kDa木质部汁液蛋白丰富

IF 1 Q3 PLANT SCIENCES

Plant Root Pub Date : 2011-01-01 DOI:10.3117/PLANTROOT.5.63

J. Furukawa, Yuta Abe, H. Mizuno, Kaoru Matsuki, Keiko Sagawa, H. Mori, H. Iwai, S. Satoh

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引用次数: 2

摘要

为了研究环境对黑杨根功能的调节作用，每年采集黑杨木质部汁液，并对汁液中的蛋白质进行分析。木质部汁液蛋白(XSP25)在冬季含量最高，分子量为25 kDa，质谱分析表明该蛋白与烟草BY-2细胞中ABA诱导的碱性分泌蛋白具有较高的相似性。利用杨树(Populus maximowiczii)全基因组序列信息，从日本自然生长杨树(Populus maximowiczii)中克隆了PmXSP25基因。PmXSP25在12月和2月在根中表达丰富，在秋季根中施用ABA后表达增强。我们认为，诱导休眠的短日照和低温促进了XSP25在根内的合成，可能是通过ABA对冬季环境的适应。

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Abscisic acid-inducible 25 kDa xylem sap protein abundant in winter poplar

To investigate the environmental regulation of root function, xylem sap was annually collected from Populus nigra and the proteins in the sap were analyzed. A 25 kDa xylem sap protein (XSP25) was found to be most abundant in the xylem sap in winter and mass spectrometry analyses showed its high similarity to abscisic acid (ABA)-inducible basic secretory protein reported in tobacco BY-2 cells. By utilizing the information of whole genome sequence of Populus, PmXSP25 was cloned from Populus maximowiczii, naturally growing poplar in Japan. The expression of PmXSP25 was abundant in root at December and February and strongly enhanced by ABA application to the autumn root. We suggest that dormancy- inducing short day length and low temperature promote the synthesis of XSP25 in root, possibly via ABA, as an adaptation to the winter environment.

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来源期刊

Plant Root PLANT SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.