穿心莲细胞壁的细胞毒活性。一项体外研究

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Medha A. Bhat, N. M. Hoskatte
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引用次数: 1

摘要

Bhat等:穿心莲的抗癌活性本研究的主要目的是评价穿心莲壁的细胞毒活性。C.B.克拉克叶甲醇提取物对癌细胞系的影响。研究穿心术叶提取物对A375(黑色素瘤)、A549(肺癌)、HCT116(结肠癌)、HeLa(宫颈)、HepG2(肝癌)、Jurkat细胞(t细胞急性淋巴母细胞白血病)、MCF7(乳腺癌)、PA1(卵巢)、PC3(前列腺)和U87MG(胶质母细胞瘤)癌细胞的体外细胞毒性。穿心莲叶提取物对所有细胞系均有细胞毒性,但对卵巢癌细胞系的细胞毒性最强(IC50=39.7µg/ml)。细胞周期和末端脱氧核苷酸转移酶dUTP末端标记实验证实穿心莲叶提取物具有细胞毒活性。采用反相高效液相色谱法对穿心莲叶提取物的有效成分进行鉴定,结果表明穿心莲内酯是其主要成分。本研究首次报道穿心莲叶提取物的抗癌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxic Activity of Andrographis lineata Wall. ex Nees: An In Vitro study
Bhat et al. : Anti-cancer Activity of Andrographis lineata var. lawii The major objective of this investigation was to evaluate the cytotoxic activity of Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke leaf methanol extract on cancer cell lines. In vitro cytotoxicity of Andrographis lineata leaf extract was evaluated against A375 (melanoma), A549 (lung), HCT116 (colon), HeLa (cervix), HepG2 (hepatoma), Jurkat cells (T-cell acute lymphoblastic leukemia), MCF7 (breast cancer), PA1 (ovary), PC3 (prostate) and U87MG (Glioblastoma) cancer cell lines. Andrographis lineata leaf extract showed cytotoxicity activity against all cell lines, however, optimum cytotoxicity was observed against the ovarian cancer cell line (IC50=39.7 µg/ml) cell line. Cell cycle and terminal deoxynucleotidyl transferase dUTP end labeling assays confirmed the cytotoxic activity of Andrographis lineata leaf extract. Reverse phase-high performance liquid chromatography analysis was performed to recognize the active ingredient in the Andrographis lineata leaf extract and the results reveal that andrographolide was the major component. This investigation is the first report of the anticancer activity of Andrographis lineata leaf extract.
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来源期刊
自引率
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审稿时长
2 months
期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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