家兔骨质疏松过程的放射学和病理化学特征

T. Todosiuk, M. Rublenko, V. Vlasenko
{"title":"家兔骨质疏松过程的放射学和病理化学特征","authors":"T. Todosiuk, M. Rublenko, V. Vlasenko","doi":"10.33245/2310-4902-2022-173-1-170-178","DOIUrl":null,"url":null,"abstract":"Various bone and joint pathology is quite common among companion animals, a significant risk factor for which is considered to be a violation of bone metabolism. The aim of the work is to determine radiological and pathochemical criteria in the dynamics of osteoporosis process in the model of rabbits. The experimental (n = 18) and control (n = 9) groups of animals were formed. Experimental osteoporosis in rabbits of the experimental group was caused by the administration of 0.4% solution of dexamethasone (4 mg / ml) (KRKA, Slovenia) for the 21st day at a dose of 1.2 mg / kg body weight 1 time / day. Control animals were injected with saline. Blood samples for biochemical studies were taken before the induction of osteoporosis and on the 7th, 14th, 21st day. Serum levels of total calcium (Ca) and phosphorus, activity of total alkaline phosphatase and its bone isoenzyme, tartrate-resistant acid phosphatase were determined. Measurements were performed with a spectrophotometer Stat Fax 4500. X-ray examinations were performed on an X-ray machine RUM-20 before the induction of osteoporosis and on the 7th, 14th, 21st day of the study. On the 14th day of the study, some areas of the spongy substance of the proximal femur have the appearance of a large-loop pattern due to the resorption of individual bone beams. There is also an expansion of the bone marrow canal due to bone resorption, thinning and emphasizing the edges of the cortical layer. On the 21st day, changes characteristic of osteoporosis were observed - low intensity of X-ray contrast shadow of bone, significant thinning of bone structure. Radiographically established changes in bone tissue correlate with biochemical parameters of rabbit blood. On the 21st day of osteoporosis induction in experimental animals, the level of total Ca decreased 2.1 times (p <0.001), and inorganic P increased 1.8 times (p <0.001), compared with normal and control animals. The level of total alkaline phosphatase and bone isoenzyme alkaline phosphatase on the 21st day of the study exceeded the performance of clinically healthy and control animals 1.1 times (p <0.001). The activity of tartrate-resistant acid phosphatase increased dynamically and on the 14th day was 30.7 ± 0.36 units / l, which is 1.1 times (p <0.001) higher than its level in clinically healthy animals, and 21st day - 1.2 times (p <0.001), respectively. The corticosteroid model of osteoporosis causes the manifestation of its radiological signs on the 14th day after induction, accompanied by hypocalcemia, hyperphosphatemia with a decrease in Ca:P and increased activity of alkaline and tartrate-resistant acid phosphatases. The complete pathochemical phase of osteoporosis occurs on the 21st day, which is manifested by a significant decrease in the ratio of phosphatases.\nKey words: secondary osteoporosis, bone fractures, corticosteroids, dexamethasone, compact and spongy bone tissue, rabbits.","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Radiological and pathochemical characteristics of osteoporosis process in rabbits\",\"authors\":\"T. Todosiuk, M. Rublenko, V. Vlasenko\",\"doi\":\"10.33245/2310-4902-2022-173-1-170-178\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Various bone and joint pathology is quite common among companion animals, a significant risk factor for which is considered to be a violation of bone metabolism. The aim of the work is to determine radiological and pathochemical criteria in the dynamics of osteoporosis process in the model of rabbits. The experimental (n = 18) and control (n = 9) groups of animals were formed. Experimental osteoporosis in rabbits of the experimental group was caused by the administration of 0.4% solution of dexamethasone (4 mg / ml) (KRKA, Slovenia) for the 21st day at a dose of 1.2 mg / kg body weight 1 time / day. Control animals were injected with saline. Blood samples for biochemical studies were taken before the induction of osteoporosis and on the 7th, 14th, 21st day. Serum levels of total calcium (Ca) and phosphorus, activity of total alkaline phosphatase and its bone isoenzyme, tartrate-resistant acid phosphatase were determined. Measurements were performed with a spectrophotometer Stat Fax 4500. X-ray examinations were performed on an X-ray machine RUM-20 before the induction of osteoporosis and on the 7th, 14th, 21st day of the study. On the 14th day of the study, some areas of the spongy substance of the proximal femur have the appearance of a large-loop pattern due to the resorption of individual bone beams. There is also an expansion of the bone marrow canal due to bone resorption, thinning and emphasizing the edges of the cortical layer. On the 21st day, changes characteristic of osteoporosis were observed - low intensity of X-ray contrast shadow of bone, significant thinning of bone structure. Radiographically established changes in bone tissue correlate with biochemical parameters of rabbit blood. On the 21st day of osteoporosis induction in experimental animals, the level of total Ca decreased 2.1 times (p <0.001), and inorganic P increased 1.8 times (p <0.001), compared with normal and control animals. The level of total alkaline phosphatase and bone isoenzyme alkaline phosphatase on the 21st day of the study exceeded the performance of clinically healthy and control animals 1.1 times (p <0.001). The activity of tartrate-resistant acid phosphatase increased dynamically and on the 14th day was 30.7 ± 0.36 units / l, which is 1.1 times (p <0.001) higher than its level in clinically healthy animals, and 21st day - 1.2 times (p <0.001), respectively. The corticosteroid model of osteoporosis causes the manifestation of its radiological signs on the 14th day after induction, accompanied by hypocalcemia, hyperphosphatemia with a decrease in Ca:P and increased activity of alkaline and tartrate-resistant acid phosphatases. The complete pathochemical phase of osteoporosis occurs on the 21st day, which is manifested by a significant decrease in the ratio of phosphatases.\\nKey words: secondary osteoporosis, bone fractures, corticosteroids, dexamethasone, compact and spongy bone tissue, rabbits.\",\"PeriodicalId\":34230,\"journal\":{\"name\":\"Naukovii visnik veterinarnoyi meditsini\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Naukovii visnik veterinarnoyi meditsini\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33245/2310-4902-2022-173-1-170-178\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Naukovii visnik veterinarnoyi meditsini","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33245/2310-4902-2022-173-1-170-178","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

各种骨骼和关节病变在伴侣动物中很常见,其中一个重要的危险因素被认为是骨骼代谢的破坏。这项工作的目的是确定放射学和病理化学标准在骨质疏松症的动态过程中,在兔模型。分为试验组(n = 18)和对照组(n = 9)。实验组家兔的实验性骨质疏松症是给药0.4%地塞米松溶液(4mg / ml) (KRKA,斯洛文尼亚),剂量为1.2 mg / kg体重1次/天,持续第21天。对照动物注射生理盐水。骨质疏松诱导前及第7、14、21天分别采血进行生化研究。测定血清总钙、总磷水平、总碱性磷酸酶及其骨同工酶、抗酒石酸酸性磷酸酶活性。用分光光度计Stat Fax 4500进行测量。骨质疏松诱导前及研究第7、14、21天在RUM-20 x线机上行x线检查。在研究的第14天,由于单个骨梁的吸收,股骨近端海绵状物质的某些区域出现大环状图案。骨髓管也因骨吸收而扩张,使皮质层边缘变薄和突出。第21天观察骨质疏松的变化特征:骨x线对比影低,骨结构明显变薄。x线摄影证实骨组织的变化与兔血液的生化参数相关。骨质疏松诱导第21天,与正常和对照动物相比,总钙水平降低了2.1倍(p <0.001),无机磷水平升高了1.8倍(p <0.001)。试验第21天总碱性磷酸酶和骨同工酶碱性磷酸酶水平是临床健康动物和对照组的1.1倍(p <0.001)。抗酒石酸酸性磷酸酶活性动态升高,第14天为30.7±0.36单位/升,是临床健康动物的1.1倍(p <0.001),第21天为1.2倍(p <0.001)。骨质疏松症皮质类固醇模型诱导后第14天出现影像学征象,伴低钙血症、高磷血症,Ca:P降低,碱性和抗酒石酸盐酸性磷酸酶活性升高。骨质疏松的完整病理化学期出现在第21天,表现为磷酸酶比例明显下降。关键词:继发性骨质疏松,骨折,皮质类固醇,地塞米松,致密和海绵状骨组织,兔子
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Radiological and pathochemical characteristics of osteoporosis process in rabbits
Various bone and joint pathology is quite common among companion animals, a significant risk factor for which is considered to be a violation of bone metabolism. The aim of the work is to determine radiological and pathochemical criteria in the dynamics of osteoporosis process in the model of rabbits. The experimental (n = 18) and control (n = 9) groups of animals were formed. Experimental osteoporosis in rabbits of the experimental group was caused by the administration of 0.4% solution of dexamethasone (4 mg / ml) (KRKA, Slovenia) for the 21st day at a dose of 1.2 mg / kg body weight 1 time / day. Control animals were injected with saline. Blood samples for biochemical studies were taken before the induction of osteoporosis and on the 7th, 14th, 21st day. Serum levels of total calcium (Ca) and phosphorus, activity of total alkaline phosphatase and its bone isoenzyme, tartrate-resistant acid phosphatase were determined. Measurements were performed with a spectrophotometer Stat Fax 4500. X-ray examinations were performed on an X-ray machine RUM-20 before the induction of osteoporosis and on the 7th, 14th, 21st day of the study. On the 14th day of the study, some areas of the spongy substance of the proximal femur have the appearance of a large-loop pattern due to the resorption of individual bone beams. There is also an expansion of the bone marrow canal due to bone resorption, thinning and emphasizing the edges of the cortical layer. On the 21st day, changes characteristic of osteoporosis were observed - low intensity of X-ray contrast shadow of bone, significant thinning of bone structure. Radiographically established changes in bone tissue correlate with biochemical parameters of rabbit blood. On the 21st day of osteoporosis induction in experimental animals, the level of total Ca decreased 2.1 times (p <0.001), and inorganic P increased 1.8 times (p <0.001), compared with normal and control animals. The level of total alkaline phosphatase and bone isoenzyme alkaline phosphatase on the 21st day of the study exceeded the performance of clinically healthy and control animals 1.1 times (p <0.001). The activity of tartrate-resistant acid phosphatase increased dynamically and on the 14th day was 30.7 ± 0.36 units / l, which is 1.1 times (p <0.001) higher than its level in clinically healthy animals, and 21st day - 1.2 times (p <0.001), respectively. The corticosteroid model of osteoporosis causes the manifestation of its radiological signs on the 14th day after induction, accompanied by hypocalcemia, hyperphosphatemia with a decrease in Ca:P and increased activity of alkaline and tartrate-resistant acid phosphatases. The complete pathochemical phase of osteoporosis occurs on the 21st day, which is manifested by a significant decrease in the ratio of phosphatases. Key words: secondary osteoporosis, bone fractures, corticosteroids, dexamethasone, compact and spongy bone tissue, rabbits.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
10 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信