紫穗槐提取物酚类化合物及抗氧化、抗菌活性的HPLC分析

B. Ivanescu, C. Lungu, L. Vlase, A. Gradinaru, C. Tuchiluş
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引用次数: 2

摘要

本研究主要对紫穗槐叶、枝和果实中乙醇含量为70% v/v的提取物进行化学和生物学分析。福林法测定的总酚含量最高,为159.5 mg/g。HPLC-MS分析表明,三种提取物中均含有阿魏酸、木犀草素和芦花苷。DPPH法测定其抗氧化活性,结果表明,果实提取物的抗氧化活性最高(EC50 = 18.8±0.2 μg/mL),其次为叶提取物(EC50 = 38.03±0.75 μg/mL),枝提取物(EC50 = 221.16±1.7 μg/mL)。琼脂盘扩散法抑菌活性测定表明,所有样品均对金黄色葡萄球菌和黄斑肌有抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS
The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 μg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 μg/mL) and branch extract (EC50 = 221.16 ± 1.7 μg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples.
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