B. Ivanescu, C. Lungu, L. Vlase, A. Gradinaru, C. Tuchiluş
{"title":"紫穗槐提取物酚类化合物及抗氧化、抗菌活性的HPLC分析","authors":"B. Ivanescu, C. Lungu, L. Vlase, A. Gradinaru, C. Tuchiluş","doi":"10.33628/jpd.2019.26.1.77","DOIUrl":null,"url":null,"abstract":"The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 μg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 μg/mL) and branch extract (EC50 = 221.16 ± 1.7 μg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples.","PeriodicalId":16754,"journal":{"name":"Journal of Plant Development","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS\",\"authors\":\"B. Ivanescu, C. Lungu, L. Vlase, A. Gradinaru, C. Tuchiluş\",\"doi\":\"10.33628/jpd.2019.26.1.77\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 μg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 μg/mL) and branch extract (EC50 = 221.16 ± 1.7 μg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples.\",\"PeriodicalId\":16754,\"journal\":{\"name\":\"Journal of Plant Development\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Development\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33628/jpd.2019.26.1.77\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33628/jpd.2019.26.1.77","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS
The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 μg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 μg/mL) and branch extract (EC50 = 221.16 ± 1.7 μg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples.