采用RT-PCR和组织病理学方法对Van省绵羊流产病原菌进行调查

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2023-01-01 DOI:10.2754/avb202392010069

Özgül Gülaydın, C. Öztürk, I. Ekin, Z. İlhan, F. İlhan

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引用次数: 0

摘要

流产给羊产业造成了巨大的经济损失。在处理流产时，确定病因是很重要的。本研究旨在鉴定绵羊流产病例中选定的重要病原菌。采用实时荧光定量PCR (RT-PCR)技术，对来自85个不同羊群的113份标本(流产羊胎105份、胎盘4份、阴道拭子4份)进行了衣原体、流产羊、布鲁氏菌、melitensis、沙门氏菌、流产羊、伯纳氏柯谢氏菌、李斯特菌、单核增生乳杆菌和弯曲杆菌的检测，所有检测结果均为阳性，并进行了病理检查。采用免疫组织化学方法对经RT-PCR检测为melitensis和L. monocytogenes阳性的胎儿组织样本进行检测。经RT-PCR检测，35份(30.9%)样品呈阳性，其中产弧菌15份(42.8%)、9份(25.7%)、5份(14.2%)、4份(11.4%)、1份(2.8%)、S. Abortusovis、C. burnetii、单核增生L.菌和弯曲杆菌1份(2.8%)。免疫组织化学也证实了该抗原的存在。结果表明，流产弧菌是绵羊流产率最高的病原菌，在流行病学调查中采取控制措施时应予以重视。

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Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods

Abortion causes significant economic losses in the sheep industry. Determination of the aetiology is important in dealing with abortions. The present study was aimed to identify selected important bacterial pathogens in the abortion cases of sheep. A total of 113 samples (105 aborted sheep foetuses, 4 placentas, and 4 vaginal swab samples) from 85 different sheep flocks were examined by real-time PCR (RT-PCR) regarding Chlamydia (C.) spp., C. abortus, Brucella (B.) spp., B. melitensis, Salmonella (S.) spp., S. Abortusovis, Coxiella (C.) burnetii, Listeria (L.) spp., L. monocytogenes, and Campylobacter spp. All cases that were found to be positive for bacterial agents by RT-PCR, were examined pathologically. Tissue samples of foetuses that were found to be positive for B. melitensis and L. monocytogenes by RT-PCR were also investigated immunohistochemically. A total of 35 (30.9%) samples were found to be positive by RT-PCR, with 15 (42.8%), 9 (25.7%), 5 (14.2%), 4 (11.4%), 1 (2.8%), and 1 (2.8%) of them being identified as C. abortus, B. melitensis, S. Abortusovis, C. burnetii, L. monocytogenes and Campylobacter spp., respectively. The presence of the antigen was confirmed also immunohistochemically in the cases with B. melitensis and L. monocytogenes. As a consequence, C. abortus was found to cause the highest rate of sheep abortion cases, which should be taken into account when implementing control measures in epidemiological investigations.

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.