聚(乳酸-羟基乙酸)酸纳米颗粒作为鱼油在伤口愈合中的递送系统

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2022-01-01 DOI:10.2754/avb202291030285

T. Komprda, Vendula Popelková, Ludmila Kosaristanova, V. Šmídová

{"title":"聚(乳酸-羟基乙酸)酸纳米颗粒作为鱼油在伤口愈合中的递送系统","authors":"T. Komprda, Vendula Popelková, Ludmila Kosaristanova, V. Šmídová","doi":"10.2754/avb202291030285","DOIUrl":null,"url":null,"abstract":"The objective of the study was to design, synthetize and characterize poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) for possible application in a cutaneous wound healing. Morphology of NPs was evaluated by transmission electron microscopy. Antimicrobial characteristics were tested using the disk diffusion method and plate count method, and cytotoxicity was evaluated by the MTT assay. Fish oil (y) was released from PLGA NPs within the time interval (x) of 96 h according to equation y = 6.2 + 0.914x. PLGA-FO NPs did not affect growth of Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) strains. No cytotoxic effect of the tested NPs on the keratinocyte cell line was observed for concentration of 1 µg/ml. PLGA-FO NPs represent an interesting alternative for wound healing due to an excellent biocompatibility and unique release profile of FO, despite their lack of antimicrobial efficiency.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Poly(lactic-co-glycolic) acid nanoparticles as a delivery system for fish oil in wound healing\",\"authors\":\"T. Komprda, Vendula Popelková, Ludmila Kosaristanova, V. Šmídová\",\"doi\":\"10.2754/avb202291030285\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of the study was to design, synthetize and characterize poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) for possible application in a cutaneous wound healing. Morphology of NPs was evaluated by transmission electron microscopy. Antimicrobial characteristics were tested using the disk diffusion method and plate count method, and cytotoxicity was evaluated by the MTT assay. Fish oil (y) was released from PLGA NPs within the time interval (x) of 96 h according to equation y = 6.2 + 0.914x. PLGA-FO NPs did not affect growth of Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) strains. No cytotoxic effect of the tested NPs on the keratinocyte cell line was observed for concentration of 1 µg/ml. PLGA-FO NPs represent an interesting alternative for wound healing due to an excellent biocompatibility and unique release profile of FO, despite their lack of antimicrobial efficiency.\",\"PeriodicalId\":7192,\"journal\":{\"name\":\"Acta Veterinaria Brno\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Veterinaria Brno\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2754/avb202291030285\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Veterinaria Brno","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2754/avb202291030285","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 2

摘要

该研究的目的是设计、合成和表征包埋鱼油的聚乳酸-羟基乙酸(PLGA)纳米颗粒(NPs)，以用于皮肤伤口愈合。透射电镜观察NPs的形态。采用纸片扩散法和平板计数法检测其抗菌特性，采用MTT法评价其细胞毒性。根据方程y = 6.2 + 0.914x，鱼油(y)在96 h的时间间隔(x)内从PLGA NPs中释放出来。PLGA-FO NPs不影响金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌(MRSA)菌株的生长。当浓度为1µg/ml时，所测NPs对角质形成细胞系无细胞毒作用。PLGA-FO NPs虽然缺乏抗菌效率，但由于其优异的生物相容性和独特的FO释放特性，因此代表了一种有趣的伤口愈合替代方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Poly(lactic-co-glycolic) acid nanoparticles as a delivery system for fish oil in wound healing

The objective of the study was to design, synthetize and characterize poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) for possible application in a cutaneous wound healing. Morphology of NPs was evaluated by transmission electron microscopy. Antimicrobial characteristics were tested using the disk diffusion method and plate count method, and cytotoxicity was evaluated by the MTT assay. Fish oil (y) was released from PLGA NPs within the time interval (x) of 96 h according to equation y = 6.2 + 0.914x. PLGA-FO NPs did not affect growth of Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) strains. No cytotoxic effect of the tested NPs on the keratinocyte cell line was observed for concentration of 1 µg/ml. PLGA-FO NPs represent an interesting alternative for wound healing due to an excellent biocompatibility and unique release profile of FO, despite their lack of antimicrobial efficiency.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.