{"title":"紫外-可见光谱分析及生长二氯代培养物中新分离物HASIL的研究","authors":"Andryan Dwisaksana, T. Tukiran","doi":"10.26740/ujc.v10n2.p121-127","DOIUrl":null,"url":null,"abstract":"Abstrak. Spektroskopi UV-vis dan FTIR merupakan salah satu teknik dalam penentuan struktur senyawa dari hasil isolasi. Penelitian ini bertujuan untuk menganalisis struktur senyawa hasil isolasi dari ekstrak diklorometana kulit batang tumbuhan Jambu Semarang menggunakan spektroskopi UV-Vis dan FTIR. Teknik isolasi senyawa yang dilakukan meliputi Kromatografi Lapis Tipis, Kromatografi Cair Vakum dan Kromatografi Kolom Gravitasi. Analisis struktur menggunakan spektroskopi UV-Vis didapatkan spektrum dengan puncak pada panjang gelombang maksimal 239 nm yang menunjukkan adanya eksitasi elektron dari π→π* yang biasa terjadi pada senyawa fenolik. Hasil analisis FTIR didapatkan adanya pita serapan pada bilangan gelombang 3542-3401 cm-1 yang menunjukkan regangan gugus OH, 2932-2857 cm-1 menunjukkan regangan gugus C-H alifatik, 877-715 cm-1 menunjukkan adanya gugus C-H aromatik, dan 1463-1378 cm-1 memperlihatkan gugus C=C aromatik. Berdasarkan analisis spektroskopiUV-Vis dan FTIR dan melihat pola serapannya, mengindikasikan bahwa senyawa hasil isolasi adalahsuatu senyawa golongan flavonoid.Kata kunci: Jambu Semarang, Isolasi, UV-Vis, FTIR \nAbstract. UV-vis and FTIR spectroscopy is one of the technic in determining the structure of compounds from the isolation. This study aimed to analyze the structure of the isolated compound from the dichloromethane extract of the stem bark of Jambu Semarang using UV-vis and FTIR spectroscopy. Compound isolation techniques included Thin Layer Chromatography, Vacuum Liquid Chromatography, and Gravity Column Chromatography. Structural analysis used UV-Vis spectroscopy obtained a spectrum with a peak at a maximum wavelength of 239 nm which indicated the electron excitation of π → π* which is common in phenolic compounds. The results of FTIR analysis showed that there was an absorption band at wave number 3542-3401 cm-1 which showed the OH group strain, 2932-2857 cm-1showed the CH group strain was aliphatic, 877-715 cm-1 indicated the CH aromatic group, and 1463 -1378 cm-1 aromatic C=C group. Based on UV-Vis and FTIR spectroscopy analysis and looking at the absorption pattern, it indicated that the isolated compound was a compound of the flavonoid group.Keywords: Jambu Semarang, Isolation, UV-Vis, FTIR","PeriodicalId":53369,"journal":{"name":"UNESA Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"ANALISIS SPEKTROSKOPI UV-VIS DAN FTIR SENYAWA HASIL ISOLASI DARI EKSTRAK DIKLOROMETANA KULIT BATANG TUMBUHAN JAMBU SEMARANG (SYZYGIUM SAMARANGENSE)\",\"authors\":\"Andryan Dwisaksana, T. Tukiran\",\"doi\":\"10.26740/ujc.v10n2.p121-127\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstrak. Spektroskopi UV-vis dan FTIR merupakan salah satu teknik dalam penentuan struktur senyawa dari hasil isolasi. Penelitian ini bertujuan untuk menganalisis struktur senyawa hasil isolasi dari ekstrak diklorometana kulit batang tumbuhan Jambu Semarang menggunakan spektroskopi UV-Vis dan FTIR. Teknik isolasi senyawa yang dilakukan meliputi Kromatografi Lapis Tipis, Kromatografi Cair Vakum dan Kromatografi Kolom Gravitasi. Analisis struktur menggunakan spektroskopi UV-Vis didapatkan spektrum dengan puncak pada panjang gelombang maksimal 239 nm yang menunjukkan adanya eksitasi elektron dari π→π* yang biasa terjadi pada senyawa fenolik. Hasil analisis FTIR didapatkan adanya pita serapan pada bilangan gelombang 3542-3401 cm-1 yang menunjukkan regangan gugus OH, 2932-2857 cm-1 menunjukkan regangan gugus C-H alifatik, 877-715 cm-1 menunjukkan adanya gugus C-H aromatik, dan 1463-1378 cm-1 memperlihatkan gugus C=C aromatik. Berdasarkan analisis spektroskopiUV-Vis dan FTIR dan melihat pola serapannya, mengindikasikan bahwa senyawa hasil isolasi adalahsuatu senyawa golongan flavonoid.Kata kunci: Jambu Semarang, Isolasi, UV-Vis, FTIR \\nAbstract. UV-vis and FTIR spectroscopy is one of the technic in determining the structure of compounds from the isolation. This study aimed to analyze the structure of the isolated compound from the dichloromethane extract of the stem bark of Jambu Semarang using UV-vis and FTIR spectroscopy. Compound isolation techniques included Thin Layer Chromatography, Vacuum Liquid Chromatography, and Gravity Column Chromatography. Structural analysis used UV-Vis spectroscopy obtained a spectrum with a peak at a maximum wavelength of 239 nm which indicated the electron excitation of π → π* which is common in phenolic compounds. The results of FTIR analysis showed that there was an absorption band at wave number 3542-3401 cm-1 which showed the OH group strain, 2932-2857 cm-1showed the CH group strain was aliphatic, 877-715 cm-1 indicated the CH aromatic group, and 1463 -1378 cm-1 aromatic C=C group. Based on UV-Vis and FTIR spectroscopy analysis and looking at the absorption pattern, it indicated that the isolated compound was a compound of the flavonoid group.Keywords: Jambu Semarang, Isolation, UV-Vis, FTIR\",\"PeriodicalId\":53369,\"journal\":{\"name\":\"UNESA Journal of Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"UNESA Journal of Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26740/ujc.v10n2.p121-127\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"UNESA Journal of Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26740/ujc.v10n2.p121-127","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
抽象。UV-vis和FTIR的分光镜技术是分离结果中化合物结构的一种技术。这项研究的目的是用uv - vies和FTIR分光镜分析从番石榴树外皮中提取的化合物结构。所做的化合物分离技术包括薄层色谱仪、液体真空色谱仪和重力柱色谱仪。结构用光谱分析UV-Vis最多239 nm波长上的获得与光谱峰显示电子游离的π→π*常见的酚化合物。FTIR的分析发现，波数3542-3401 cm-1中有条纹，显示星系团哦，2932-2857 cm1显示C- h alifatic, 877-715 cm1显示C- h芳香星系团，1463-1378 cm1显示C=C芳香星系团。根据对光谱的分析和FTIR的观察，分离出的化合物表明是一种类黄酮。关键词:番石榴三宝朗、孤立、UV-Vis、FTIR Abstract。UV-vis和FTIR - scoscopy是确定自隔离的技术之一。这一研究表明，利用UV-vis和FTIR - scoscopy，分析了二氯甲烷从二甲抽出来的凝聚聚物的结构。合成合成技术包括层层的色素学、真空学和重力合成色素学。结构分析过去UV-Vis spectroscopy获得a频谱与峰at a maximum wavelength电子excitation》239 nm的哪种indicatedπ→π*,这是普通的酚compounds。FTIR分析的结果表明，在wave number 3542-3401 cdn -1中有一个被骂的乐队基于UV-Vis和FTIR频谱分析，并查看衰减模式，其本质是孤立的化合物是一组黄酮。小字:番石榴，孤立，UV-Vis, FTIR
ANALISIS SPEKTROSKOPI UV-VIS DAN FTIR SENYAWA HASIL ISOLASI DARI EKSTRAK DIKLOROMETANA KULIT BATANG TUMBUHAN JAMBU SEMARANG (SYZYGIUM SAMARANGENSE)
Abstrak. Spektroskopi UV-vis dan FTIR merupakan salah satu teknik dalam penentuan struktur senyawa dari hasil isolasi. Penelitian ini bertujuan untuk menganalisis struktur senyawa hasil isolasi dari ekstrak diklorometana kulit batang tumbuhan Jambu Semarang menggunakan spektroskopi UV-Vis dan FTIR. Teknik isolasi senyawa yang dilakukan meliputi Kromatografi Lapis Tipis, Kromatografi Cair Vakum dan Kromatografi Kolom Gravitasi. Analisis struktur menggunakan spektroskopi UV-Vis didapatkan spektrum dengan puncak pada panjang gelombang maksimal 239 nm yang menunjukkan adanya eksitasi elektron dari π→π* yang biasa terjadi pada senyawa fenolik. Hasil analisis FTIR didapatkan adanya pita serapan pada bilangan gelombang 3542-3401 cm-1 yang menunjukkan regangan gugus OH, 2932-2857 cm-1 menunjukkan regangan gugus C-H alifatik, 877-715 cm-1 menunjukkan adanya gugus C-H aromatik, dan 1463-1378 cm-1 memperlihatkan gugus C=C aromatik. Berdasarkan analisis spektroskopiUV-Vis dan FTIR dan melihat pola serapannya, mengindikasikan bahwa senyawa hasil isolasi adalahsuatu senyawa golongan flavonoid.Kata kunci: Jambu Semarang, Isolasi, UV-Vis, FTIR
Abstract. UV-vis and FTIR spectroscopy is one of the technic in determining the structure of compounds from the isolation. This study aimed to analyze the structure of the isolated compound from the dichloromethane extract of the stem bark of Jambu Semarang using UV-vis and FTIR spectroscopy. Compound isolation techniques included Thin Layer Chromatography, Vacuum Liquid Chromatography, and Gravity Column Chromatography. Structural analysis used UV-Vis spectroscopy obtained a spectrum with a peak at a maximum wavelength of 239 nm which indicated the electron excitation of π → π* which is common in phenolic compounds. The results of FTIR analysis showed that there was an absorption band at wave number 3542-3401 cm-1 which showed the OH group strain, 2932-2857 cm-1showed the CH group strain was aliphatic, 877-715 cm-1 indicated the CH aromatic group, and 1463 -1378 cm-1 aromatic C=C group. Based on UV-Vis and FTIR spectroscopy analysis and looking at the absorption pattern, it indicated that the isolated compound was a compound of the flavonoid group.Keywords: Jambu Semarang, Isolation, UV-Vis, FTIR
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