利用SSR技术评价云南马铃薯品种的遗传多样性

IF 0.5 4区生物学 Q4 PLANT SCIENCES

Acta Biologica Cracoviensia Series Botanica Pub Date : 2014-09-12 DOI:10.2478/ABCSB-2014-0003

Hong-Ying Liao, Huachun Guo

{"title":"利用SSR技术评价云南马铃薯品种的遗传多样性","authors":"Hong-Ying Liao, Huachun Guo","doi":"10.2478/ABCSB-2014-0003","DOIUrl":null,"url":null,"abstract":"The genetic diversity of potato cultivars collected from Yunnan Province was evaluated using 24 pairs of SSR markers. SSR analysis of 24 pairs of primers showed varying degrees of polymorphism among the 85 cultivars: 297 of the 304 bands were polymorphic. The primers yielded between 5 (STM2028) and 19 (StI029) bands (mean 12). The ratio of polymorphic bands ranged from 83.33% to 100% (mean 97.75%). Polymorphism information content (PIC) varied from 69.31% to 93.67% (mean 86.47%). Genetic similarity ranged from 0.5987 to 0.7632, indicating relatively low genetic diversity in the potato cultivars from Yunnan Province. Cluster analysis by UPGMA and PCA clearly delineated the genetic relationships of all cultivars; 83 of the 85 cultivars could be discriminated by only two pairs of primers, STM0030 and STM1104. The high polymorphism and good resolution of the primers used in this study make them good tools for discriminating potato cultivars.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2014-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/ABCSB-2014-0003","citationCount":"26","resultStr":"{\"title\":\"USING SSR TO EVALUATE THE GENETIC DIVERSITY OF POTATO CULTIVARS FROM YUNNAN PROVINCE (SW CHINA)\",\"authors\":\"Hong-Ying Liao, Huachun Guo\",\"doi\":\"10.2478/ABCSB-2014-0003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The genetic diversity of potato cultivars collected from Yunnan Province was evaluated using 24 pairs of SSR markers. SSR analysis of 24 pairs of primers showed varying degrees of polymorphism among the 85 cultivars: 297 of the 304 bands were polymorphic. The primers yielded between 5 (STM2028) and 19 (StI029) bands (mean 12). The ratio of polymorphic bands ranged from 83.33% to 100% (mean 97.75%). Polymorphism information content (PIC) varied from 69.31% to 93.67% (mean 86.47%). Genetic similarity ranged from 0.5987 to 0.7632, indicating relatively low genetic diversity in the potato cultivars from Yunnan Province. Cluster analysis by UPGMA and PCA clearly delineated the genetic relationships of all cultivars; 83 of the 85 cultivars could be discriminated by only two pairs of primers, STM0030 and STM1104. The high polymorphism and good resolution of the primers used in this study make them good tools for discriminating potato cultivars.\",\"PeriodicalId\":45465,\"journal\":{\"name\":\"Acta Biologica Cracoviensia Series Botanica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2014-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.2478/ABCSB-2014-0003\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Biologica Cracoviensia Series Botanica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.2478/ABCSB-2014-0003\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biologica Cracoviensia Series Botanica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2478/ABCSB-2014-0003","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 26

摘要

利用24对SSR标记对云南省马铃薯品种的遗传多样性进行了评价。对24对引物的SSR分析显示，85个品种间存在不同程度的多态性，304条引物中有297条为多态性。引物产生5个(STM2028) ~ 19个(StI029)条带(平均12个)。多态性频带比例为83.33% ~ 100%(平均97.75%)。多态性信息含量(PIC)变化范围为69.31% ~ 93.67%，平均为86.47%。遗传相似度为0.5987 ~ 0.7632，表明云南马铃薯品种遗传多样性相对较低。UPGMA聚类分析和PCA聚类分析清晰地描述了各品种的亲缘关系;85个品种中有83个品种仅用STM0030和STM1104两对引物就能识别。引物多态性高，分辨率高，是马铃薯品种鉴别的良好工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

USING SSR TO EVALUATE THE GENETIC DIVERSITY OF POTATO CULTIVARS FROM YUNNAN PROVINCE (SW CHINA)

The genetic diversity of potato cultivars collected from Yunnan Province was evaluated using 24 pairs of SSR markers. SSR analysis of 24 pairs of primers showed varying degrees of polymorphism among the 85 cultivars: 297 of the 304 bands were polymorphic. The primers yielded between 5 (STM2028) and 19 (StI029) bands (mean 12). The ratio of polymorphic bands ranged from 83.33% to 100% (mean 97.75%). Polymorphism information content (PIC) varied from 69.31% to 93.67% (mean 86.47%). Genetic similarity ranged from 0.5987 to 0.7632, indicating relatively low genetic diversity in the potato cultivars from Yunnan Province. Cluster analysis by UPGMA and PCA clearly delineated the genetic relationships of all cultivars; 83 of the 85 cultivars could be discriminated by only two pairs of primers, STM0030 and STM1104. The high polymorphism and good resolution of the primers used in this study make them good tools for discriminating potato cultivars.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Acta Biologica Cracoviensia Series Botanica 生物-植物科学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ACTA BIOLOGICA CRACOVIENSIA Series Botanica is an English-language journal founded in 1958, devoted to plant anatomy and morphology, cytology, genetics, embryology, tissue culture, physiology, biochemistry, biosystematics, molecular phylogenetics and phylogeography, as well as phytochemistry. It is published twice a year.