L. Mala, K. Lalouckova, P. Hovorková, E. Skřivanová
{"title":"嗜水气单胞菌对中链脂肪酸及其单酯的敏感性","authors":"L. Mala, K. Lalouckova, P. Hovorková, E. Skřivanová","doi":"10.2478/sab-2020-0008","DOIUrl":null,"url":null,"abstract":"Abstract Medium-chain fatty acids (MCFAs) and their monoesters were tested for their antibacterial activity against the Gram-negative pathogen Aeromonas hydrophila. The antimicrobial effect was evaluated at two temperatures (4 °C and 37 °C) using a standardized microdilution method in a 96-well microtitration plate. The minimum inhibitory concentrations of selected MCFAs were determined as the lowest concentration limiting the growth of A. hydrophila in wells compared to a positive control of ≥ 80%. The results indicated that the most effective compound against A. hydrophila was sucrose monocaprate after incubation at 37 °C (0.625 mg ml−1), whereas monocaprylin was the most effective compound after incubation at 4 °C (1.25 mg ml−1). Free MCFAs showed no antibacterial effects towards this bacterium. Low solubility and sensory properties could limit the use of fatty acids in aquatic environment, which should be the subject of further studies.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Susceptibility of Aeromonas hydrophila to Medium-Chain Fatty Acids and their Monoesters\",\"authors\":\"L. Mala, K. Lalouckova, P. Hovorková, E. Skřivanová\",\"doi\":\"10.2478/sab-2020-0008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Medium-chain fatty acids (MCFAs) and their monoesters were tested for their antibacterial activity against the Gram-negative pathogen Aeromonas hydrophila. The antimicrobial effect was evaluated at two temperatures (4 °C and 37 °C) using a standardized microdilution method in a 96-well microtitration plate. The minimum inhibitory concentrations of selected MCFAs were determined as the lowest concentration limiting the growth of A. hydrophila in wells compared to a positive control of ≥ 80%. The results indicated that the most effective compound against A. hydrophila was sucrose monocaprate after incubation at 37 °C (0.625 mg ml−1), whereas monocaprylin was the most effective compound after incubation at 4 °C (1.25 mg ml−1). Free MCFAs showed no antibacterial effects towards this bacterium. Low solubility and sensory properties could limit the use of fatty acids in aquatic environment, which should be the subject of further studies.\",\"PeriodicalId\":53537,\"journal\":{\"name\":\"Scientia Agriculturae Bohemica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Agriculturae Bohemica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/sab-2020-0008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Agriculturae Bohemica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/sab-2020-0008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Susceptibility of Aeromonas hydrophila to Medium-Chain Fatty Acids and their Monoesters
Abstract Medium-chain fatty acids (MCFAs) and their monoesters were tested for their antibacterial activity against the Gram-negative pathogen Aeromonas hydrophila. The antimicrobial effect was evaluated at two temperatures (4 °C and 37 °C) using a standardized microdilution method in a 96-well microtitration plate. The minimum inhibitory concentrations of selected MCFAs were determined as the lowest concentration limiting the growth of A. hydrophila in wells compared to a positive control of ≥ 80%. The results indicated that the most effective compound against A. hydrophila was sucrose monocaprate after incubation at 37 °C (0.625 mg ml−1), whereas monocaprylin was the most effective compound after incubation at 4 °C (1.25 mg ml−1). Free MCFAs showed no antibacterial effects towards this bacterium. Low solubility and sensory properties could limit the use of fatty acids in aquatic environment, which should be the subject of further studies.