埃及大麦抗叶条病qtl定位

S. Adawy, R. Ismail, M. Sakr
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引用次数: 0

摘要

大麦叶条病是一种由稻瘟病菌(Pyrenophora graminea)引起的重要的种子传播病害,在北欧和地中海大麦区也有发生。在本研究中,构建了一个F2和7个LSLs的QTL图谱,分布在8个连锁群上,覆盖了大麦基因组的2099.4 cM。LG2 (2H)的连锁群长度为117.8 cM, LG6 (6H)的连锁群长度为482.9 cM,平均长度为262.4 cM。根据所使用的锚点SSR标记,仅将4个连锁群分配到染色体上,其中LG2、LG5、LG6和LG7分别分配到2H、5H、6H和7H染色体上。采用单点分析鉴定控制叶条严重程度和株高性状的基因组区域。共鉴定出40个与病重(DS)和株高(PH)性状相关的QTL(病重20个,株高20个)。这项工作代表了大麦群体的第一个遗传连锁图谱,该图谱来源于At20和At29之间的种内杂交,显示了大麦中与疾病严重程度和株高性状相关的染色体区域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MAPPING OF QTLs CONTROLLING RESISTANCE TO LEAF STRIPE (Pyrenophora graminea) IN EGYPTIAN BARLEY
Leaf stripe in barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. In the present work, a QTL map for F2 barley and 7 LSLs) distributed on 8 linkage groups and spanning 2099.4 cM of the barley genome. The size of linkage groups varied from 117.8 cM for LG2 (2H) to 482.9 cM for LG6 (6H) with an average length of 262.4 cM. Based on the used anchor SSR markers, only four linkage groups were assigned to chromosomes, where LG2, LG5, LG6 and LG7 were assigned to chromosomes 2H, 5H, 6H and 7H, respectively. Single point analysis was used to identify genomic regions controlling the leaf stripe severity and plant height traits. A total of 40 QTL were identified for the disease severity (DS) and plant height (PH) traits (20 QTL for disease severity and 20 QTL for plant height). This work represents the first genetic linkage map for barley population derived from an intraspecific cross between At20 and At29 showing chromosomal regions associated with disease severity and plant height traits in barley.
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