{"title":"鳞翅目棉铃虫液泡atp酶B、C亚基的靶向研究麦蛾科)","authors":"A. Mohammed","doi":"10.21608/EJGC.2013.10192","DOIUrl":null,"url":null,"abstract":"The vacuolar proton pump, V-ATPase, is located in the apical cell membranes of the goblet cells within the midgut of lepidopteran larvae. It plays a role in amino acid absorption, by energiz-ing the plasma membrane through pump-ing H+ ions/proton into the goblet lumen. The full transcripts of V-ATPase subunit B and C were sequenced from the midgut of pink bollworm larvae. We used RNAi to determine the efficacy of dsRNA on silencing genes encoding B and C subu-nits. Larval injection with dsRNAs target-ing subunits B and C caused larval mortal-ity of 30.77% and 25.68%, respectively.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"365-378"},"PeriodicalIF":0.0000,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"TARGETING THE VACUOLAR ATPase SUBUNITS B AND C IN PINK BOLLWORM, Pectinophora gossypiella (Saunders) (Lepidoptera; Gelechiidae)\",\"authors\":\"A. Mohammed\",\"doi\":\"10.21608/EJGC.2013.10192\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The vacuolar proton pump, V-ATPase, is located in the apical cell membranes of the goblet cells within the midgut of lepidopteran larvae. It plays a role in amino acid absorption, by energiz-ing the plasma membrane through pump-ing H+ ions/proton into the goblet lumen. The full transcripts of V-ATPase subunit B and C were sequenced from the midgut of pink bollworm larvae. We used RNAi to determine the efficacy of dsRNA on silencing genes encoding B and C subu-nits. Larval injection with dsRNAs target-ing subunits B and C caused larval mortal-ity of 30.77% and 25.68%, respectively.\",\"PeriodicalId\":31811,\"journal\":{\"name\":\"Egyptian Journal of Genetics and Cytology\",\"volume\":\"42 1\",\"pages\":\"365-378\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Genetics and Cytology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/EJGC.2013.10192\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Genetics and Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EJGC.2013.10192","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
TARGETING THE VACUOLAR ATPase SUBUNITS B AND C IN PINK BOLLWORM, Pectinophora gossypiella (Saunders) (Lepidoptera; Gelechiidae)
The vacuolar proton pump, V-ATPase, is located in the apical cell membranes of the goblet cells within the midgut of lepidopteran larvae. It plays a role in amino acid absorption, by energiz-ing the plasma membrane through pump-ing H+ ions/proton into the goblet lumen. The full transcripts of V-ATPase subunit B and C were sequenced from the midgut of pink bollworm larvae. We used RNAi to determine the efficacy of dsRNA on silencing genes encoding B and C subu-nits. Larval injection with dsRNAs target-ing subunits B and C caused larval mortal-ity of 30.77% and 25.68%, respectively.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
