Ribeiro Menossi, Leandro da Rin de Sandre, Giancarlo de Souza Dias, Michelle da Cunha Abreu Xavier, A. D. de Almeida, E. D. da Silva, A. Maiorano, Rafael Firmani Perna, Sergio Andres Villalba Morales
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引用次数: 0
摘要
低聚果糖是一种果糖低聚物,作为益生元糖,对人体健康和营养有益。它们的产生是通过附着在微生物细胞上的果糖基转移酶(FTase, E.C.2.4.1.9)催化蔗糖分子的转果糖基化反应进行的。本研究的目的是研究戊二醛交联米曲霉IPT-301细胞的制备、酶活性和稳定性,该细胞作为生物催化剂在填充床反应器(PBR)中用于蔗糖的转果糖基化反应,以生产FOS。交联制备的生物催化剂在转速为200转/分、转速为45 min时转果糖基化活性最高。C,流速为3ml min-1至5ml min-1,蔗糖浓度为473 g L-1和500 g L-1。采用Michaelis-Menten模型描述酶促动力学。最后,生物催化剂在连续和不连续流动的PBR中反应12小时的恒定AT分别约为75 U g-1和300 U g-1。这些结果表明,戊二醛交联的m.o yzae IPT-301细胞作为PBR反应器中连续生产FOS的非均相生物催化剂具有很高的潜力。
Crosslinked whole cells for the sucrose transfructosylation reaction in a continuous reactor
Fructooligosaccharides (FOS) are fructose oligomers that are beneficial to human health and nutrition for being prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT-301 cells used as biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (AT) was presented by the biocatalyst prepared by crosslinking at 200 rpm and 45 min. The highest AT in the PBR were obtained at 50?C, flow rates from 3 mL min-1 to 5 mL min-1 and sucrose concentrations of 473 g L-1 and 500 g L-1. The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant AT of approximately 75 U g-1 and 300 U g-1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehyde-crosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors.
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