蛋白质组学分析揭示了经典和高致病性肺炎克雷伯菌分离株代谢途径的改变

Pub Date : 2022-01-01 DOI:10.2298/abs220613022y

Hui Yu, Lixia Zhang, Rina Su, Hai-ying Hu, Zhanli Wang

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引用次数: 0

摘要

肺炎克雷伯菌是一种机会性病原体，可引起广泛的感染。高毒力肺炎克雷伯菌(hvKp)的出现和传播在几个微生物学方面似乎不同于经典肺炎克雷伯菌(cKp)，是一个紧迫的全球威胁。然而，hvKp的毒力特征及其与cKp的区别尚不清楚。本工作旨在利用蛋白质组学研究蛋白质表达特征与高毒力之间的关系。结果显示，与cKp分离株相比，hvKp分离株有185个蛋白表达上调，266个蛋白表达下调。根据基因本体(GO)富集和京都基因与基因组百科全书(KEGG)途径对差异表达蛋白进行功能分类。这些蛋白主要与代谢相关，这表明hvKp分离株代谢途径的变化可能部分促成了高毒力。

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Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis

Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence.

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