紫菀在氧化锌纳米颗粒合成及诱导乳腺癌细胞凋亡中的作用

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Zahra shamasi, A. Es‐haghi, M. E. T. Yazdi, M. Amiri, Masoud Homayouni-Tabrizi
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引用次数: 36

摘要

目标:如今,纳米技术在解决癌症治疗方面取得了巨大的成功,并创造了一个新的跨学科研究领域,包括各种科学,如生物学,化学和医学。细胞凋亡是调节细胞生长和增殖、维持机体发育和总体稳态的一种保守和受控制的策略。氧化锌纳米粒子(ZnO-NPs)是目前应用最广泛的纳米粒子。本研究旨在评价红檗水提物合成的ZnO-NPs对MCF7乳腺癌细胞株的凋亡诱导作用。材料与方法:以红草提取物为原料合成氧化锌纳米颗粒,并采用动态光散射(DLS)、场发射扫描电镜(FESEM)和x射线衍射分析(XRD)等方法对其进行表征。采用Hoechst染色、吖啶橙/碘化Propodium染色及流式细胞术检测细胞凋亡。结果:本研究结果表明,用白荆提取物生物合成的ZnO-NPs粒径约为40 nm,呈球形。Hoechst染色、吖啶橙/碘化Propodium染色及流式细胞术结果显示,生物合成ZnO-NPs能有效且剂量依赖性地诱导MCF7乳腺癌细胞凋亡。结论:因此,用白芷水提物生物合成ZnO-NPs可用于包括癌症在内的多种疾病的治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Rubia tinctorum in the synthesis of zinc oxide nanoparticles and apoptosis induction in breast cancer cell line
Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line. Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry. Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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审稿时长
12 weeks
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