Zahra shamasi, A. Es‐haghi, M. E. T. Yazdi, M. Amiri, Masoud Homayouni-Tabrizi
{"title":"紫菀在氧化锌纳米颗粒合成及诱导乳腺癌细胞凋亡中的作用","authors":"Zahra shamasi, A. Es‐haghi, M. E. T. Yazdi, M. Amiri, Masoud Homayouni-Tabrizi","doi":"10.22038/NMJ.2021.08.07","DOIUrl":null,"url":null,"abstract":"Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line. Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry. Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"8 1","pages":"65-72"},"PeriodicalIF":1.4000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"36","resultStr":"{\"title\":\"Role of Rubia tinctorum in the synthesis of zinc oxide nanoparticles and apoptosis induction in breast cancer cell line\",\"authors\":\"Zahra shamasi, A. Es‐haghi, M. E. T. Yazdi, M. Amiri, Masoud Homayouni-Tabrizi\",\"doi\":\"10.22038/NMJ.2021.08.07\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line. Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry. Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.\",\"PeriodicalId\":18933,\"journal\":{\"name\":\"Nanomedicine Journal\",\"volume\":\"8 1\",\"pages\":\"65-72\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"36\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/NMJ.2021.08.07\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NANOSCIENCE & NANOTECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2021.08.07","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
Role of Rubia tinctorum in the synthesis of zinc oxide nanoparticles and apoptosis induction in breast cancer cell line
Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line. Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry. Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.