用高效液相色谱-质谱联用法测定纸上干血迹中的磷脂酰乙醇:化学依赖者酒精消费的评价

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Quimica Nova Pub Date : 2023-02-01 DOI:10.21577/0100-4042.20170968

Mariane Tegner, Fernanda Guterres, Isabela Ott, Lidiane da Motta, Deise Schmitz, M. Perassolo, Rafael Linden, Marina Antunes

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引用次数: 0

摘要

uhplc-ms / ms法测定干血斑中磷脂酰乙醇:化学依赖者酒精消耗的评价。我们开发并验证了一种UHPLC-MS/MS定量干血斑(DBS)中磷脂酰乙醇(PEth)的方法。样品制备为液体萃取，在Acquity C8柱上进行色谱分离。流动相为4 mM乙酸铵在水(A)和乙腈(B)中的比例为24:76 (v/v)。总分析时间为6分钟，其中PEth保留时间为3.36 min，磷脂酰丙醇保留时间为4.13 min。方法在10 ~ 3000 ng mL-1范围内呈线性关系，特异度高，准确度高。该分析物在-20 ~ 45°C的DBS中保存21天稳定。矩阵效应用内标补偿(-5.21% ~ + 6.09%)。该方法应用于25名化学依赖者的DBS酒精消耗评估。PEth浓度范围为14.5 ~ 2380.8 ng mL-1，与自我报告酒精消耗审计评分显著相关(r=0.41)。

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DETERMINAÇÃO DE FOSFATIDILETANOL EM MANCHAS DE SANGUE SECO EM PAPEL POR UHPLC-MS/MS: AVALIAÇÃO DO CONSUMO DE ÁLCOOL EM DEPENDENTES QUÍMICOS

DETERMINATION OF PHOSPHATIDYLETANOL IN DRIED BLOOD SPOT BY UHPLC-MS/MS: EVALUATION OF ALCOHOL CONSUMPTION IN CHEMICAL DEPENDENTS. We developed and validated an UHPLC-MS/MS method for the quantification of phosphatidylethanol (PEth) in dried blood spots (DBS). Sample preparation was a liquid extraction and chromatographic separation was performed in an Acquity C8 column. The mobile phases were 4 mM ammonium acetate in water (A) and acetonitrile (B) at 24:76 (v/v). The total analytical run time was 6 minutes, with retention time of 3.36 min for PEth and 4.13 min for phosphatidylpropanol. The method was linear from 10 to 3000 ng mL-1, specific, with, precise and accurate. The analyte was stable in DBS stored from -20 to 45 °C for 21 days. Matrix effect was compensated with the internal standard (-5.21% to + 6.09%). The method was applied in the evaluation of alcohol consumption in DBS from 25 chemical dependents. The PEth concentrations ranged from 14.5 to 2380.8 ng mL-1, with significant correlation with the self-report alcohol consumption AUDIT scores (r=0.41).

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来源期刊

Quimica Nova 化学-化学综合

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

2-4 weeks

期刊介绍： Química Nova publishes in portuguese, spanish and english, original research articles, revisions, technical notes and articles about education in chemistry. All the manuscripts submitted to QN are evaluated by, at least, two reviewers (from Brazil and abroad) of recognized expertise in the field of chemistry involved in the manuscript. The Editorial Council can be eventually asked to review manuscripts. Editors are responsible for the final edition of QN.