与人类CST2基因相关的Sac I限制性片段长度多态性(RFLP)。

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Bulletin of Tokyo Dental College Pub Date : 2002-02-01 DOI:10.2209/TDCPUBLICATION.43.41

K. Minaguchi, T. Kiriyama, E. Saitoh, S. Isemura, K. Sanada

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引用次数: 0

摘要

利用限制性内切酶Sac i在日本人群中检测了胱抑素基因(CST)家族的限制性内切片段长度多态性(RFLP)，该多态性位点位于CST2基因3′端0.9 kb处，与CST1基因外显子2探针杂交发现了条带大小分别为3.5 kb和8.3 kb的两个等位基因多态性。日本人群3.5 kb等位基因频率为0.326,8.3 kb等位基因频率为0.674 (n = 86)。该多态性的表型与先前报道的电泳胱抑素SA蛋白表型无关。

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Sac I Restriction Fragment Length Polymorphism (RFLP) related to the human CST2 gene.

Restriction Fragment Length Polymorphism (RFLP) related to cystatin gene (CST) family was detected in the Japanese population by using restriction enzyme Sac I. A polymorphic site, located at 0.9 kb from the 3' end of the CST2 gene, revealed a two allele polymorphism with band sizes of 3.5 kb and 8.3 kb by hybridization with probe including exon 2 of the CST1 gene. The gene frequencies in the Japanese population were 0.326 for 3.5 kb allele and 0.674 for 8.3 kb allele (n = 86). The phenotypes of the polymorphism showed no association with the previously reported electrophoretic cystatin SA protein phenotypes.

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来源期刊

Bulletin of Tokyo Dental College DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

0.90

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0.00%

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期刊介绍： The bulletin of Tokyo Dental collegue is principally for the publication of original contributions to multidisciplinary research in dentistry.