两种测定芽孢杆菌拮抗活性方法的比较

Q3 Multidisciplinary

Walailak Journal of Science and Technology Pub Date : 2011-11-13 DOI:10.2004/WJST.V5I2.86

M. Lertcanawanichakul, Songtham Sawangnop

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引用次数: 74

摘要

在本研究中，我们旨在通过交叉条纹法和琼脂孔扩散法的比较，确定各种芽孢杆菌对革兰氏(+)和革兰氏(-)细菌代表(金黄色葡萄球菌TISTR 517和大肠杆菌TISTR 887)的拮抗作用。两种方法对金黄色葡萄球菌TISTR 517的抑制效果均优于大肠杆菌TISTR 887。有趣的是，在交叉条纹法的情况下，这两种指示菌的生长都被本研究中使用的芽孢杆菌明显抑制。交叉条纹法适用于芽孢杆菌拮抗效果的初步评价。

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A Comparison of Two Methods Used for Measuring the Antagonistic Activity of Bacillus Species

In this study, we have aimed to determine antagonistic effects of various Bacillus against representatives of Gram (+) and Gram (-) bacteria, ( Staphylococcus aureus TISTR 517 and Escherichia coli TISTR 887) with a comparison between the cross streak method and agar well diffusion method. Both methods used in the experiment gave better inhibition results on the S. aureus TISTR 517 compared to the E. coli TISTR 887. Interestingly, in the case of the cross streak method, both indicator bacteria were clearly inhibited in their growth by the Bacillus species used in this study. The cross streak method was suitable for a preliminary assessment of the antagonistic effects of Bacillus species.

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来源期刊

Walailak Journal of Science and Technology Multidisciplinary-Multidisciplinary

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： The Walailak Journal of Science and Technology (Walailak J. Sci. & Tech. or WJST), is a peer-reviewed journal covering all areas of science and technology, launched in 2004. It is published 12 Issues (Monthly) by the Institute of Research and Innovation of Walailak University. The scope of the journal includes the following areas of research : - Natural Sciences: Biochemistry, Chemical Engineering, Chemistry, Materials Science, Mathematics, Molecular Biology, Physics and Astronomy. -Life Sciences: Allied Health Sciences, Biomedical Sciences, Dentistry, Genetics, Immunology and Microbiology, Medicine, Neuroscience, Nursing, Pharmaceutics, Psychology, Public Health, Tropical Medicine, Veterinary. -Applied Sciences: Agricultural, Aquaculture, Biotechnology, Computer Science, Cybernetics, Earth and Planetary, Energy, Engineering, Environmental, Food Science, Information Technology, Meat Science, Nanotechnology, Plant Sciences, Systemics