Primer1:引物设计Web服务

Q3 Computer Science

Open Bioinformatics Journal Pub Date : 2012-11-30 DOI:10.2174/1875036201206010055

A. Collins, X. Ke

{"title":"Primer1:引物设计Web服务","authors":"A. Collins, X. Ke","doi":"10.2174/1875036201206010055","DOIUrl":null,"url":null,"abstract":"Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html","PeriodicalId":38956,"journal":{"name":"Open Bioinformatics Journal","volume":"6 1","pages":"55-58"},"PeriodicalIF":0.0000,"publicationDate":"2012-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"112","resultStr":"{\"title\":\"Primer1: Primer Design Web Service for Tetra-Primer ARMS-PCR\",\"authors\":\"A. Collins, X. Ke\",\"doi\":\"10.2174/1875036201206010055\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html\",\"PeriodicalId\":38956,\"journal\":{\"name\":\"Open Bioinformatics Journal\",\"volume\":\"6 1\",\"pages\":\"55-58\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-11-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"112\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Bioinformatics Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1875036201206010055\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Bioinformatics Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1875036201206010055","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Computer Science","Score":null,"Total":0}

引用次数: 112

摘要

四引物ARMS-PCR作为一种低成本的单次PCR检测方法被广泛使用，不需要PCR后操作。成功引物的设计取决于许多变量，如熔融温度、GC含量、互补性和错配碱基的选择。引物的最佳选择可以用一个程序来自动地实现，该程序对给定序列的候选引物进行评估。Primer1软件最初是用于凝胶电泳的限制性内切片段长度多态性分析。然而，最近的应用程序已经更加多样化，在这里回顾，我们提出了Primer1软件的概述，用于引物设计和网络服务。我们已经更新了Primer1程序，并提供了更完整的实现细节。我们也提供测试数据和输出。该程序现在可以在一个新的、高效的LAMP网络服务上使用:http://primer1.soton.ac.uk/primer1.html

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Primer1: Primer Design Web Service for Tetra-Primer ARMS-PCR

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Open Bioinformatics Journal Computer Science-Computer Science (miscellaneous)

CiteScore

2.40

自引率

0.00%

发文量

期刊介绍： The Open Bioinformatics Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, letters, clinical trial studies and guest edited single topic issues in all areas of bioinformatics and computational biology. The coverage includes biomedicine, focusing on large data acquisition, analysis and curation, computational and statistical methods for the modeling and analysis of biological data, and descriptions of new algorithms and databases. The Open Bioinformatics Journal, a peer reviewed journal, is an important and reliable source of current information on the developments in the field. The emphasis will be on publishing quality articles rapidly and freely available worldwide.