模拟微重力条件下注射必需磷脂后小鼠卵母细胞细胞骨架蛋白含量的变化

Q4 Biochemistry, Genetics and Molecular Biology

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Pub Date : 2023-01-01 DOI:10.21687/0233-528x-2023-57-1-44-48

М.А. Sventitskaya, I. V. Ogneva

{"title":"模拟微重力条件下注射必需磷脂后小鼠卵母细胞细胞骨架蛋白含量的变化","authors":"М.А. Sventitskaya, I. V. Ogneva","doi":"10.21687/0233-528x-2023-57-1-44-48","DOIUrl":null,"url":null,"abstract":"The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CHANGE IN THE CONTENT OF CYTOSKELETAL PROTEINS IN MOUSE OOCYTES AFTER INJECTION OF ESSENTIAL PHOSPHOLIPIDS IN THE CONDITION OF MODELED MICROGRAVITY\",\"authors\":\"М.А. Sventitskaya, I. V. Ogneva\",\"doi\":\"10.21687/0233-528x-2023-57-1-44-48\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.\",\"PeriodicalId\":8683,\"journal\":{\"name\":\"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21687/0233-528x-2023-57-1-44-48\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21687/0233-528x-2023-57-1-44-48","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

摘要

本文评价了不同剂量的必需磷脂对在微重力模拟器上培养的小鼠卵母细胞微管和微丝细胞骨架的影响。免疫细胞化学法测定乙酰化α -微管蛋白和β -肌动蛋白总含量。模拟微重力培养后，乙酰化α -微管蛋白的相对含量降低，而β -肌动蛋白的含量保持不变。在再现微重力影响的条件下，注射必需磷脂(350 mg/kg体重)可使该参数恢复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

CHANGE IN THE CONTENT OF CYTOSKELETAL PROTEINS IN MOUSE OOCYTES AFTER INJECTION OF ESSENTIAL PHOSPHOLIPIDS IN THE CONDITION OF MODELED MICROGRAVITY

The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

0.60

自引率

0.00%

发文量