运动约束21天后体位和运动肌肉力学特性和肌球蛋白表型的变化

Q4 Biochemistry, Genetics and Molecular Biology

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Pub Date : 2022-01-01 DOI:10.21687/0233-528x-2022-56-4-70-76

S. Belova, S.A. Tyganova, B. Shenkman

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引用次数: 0

摘要

研究了Wistar大鼠在小笼运动约束21 d后体位肌和运动肌的主动力学特性。机械离体检测右腿比目鱼肌和EDL;从左腿提取的RNA用于测定各种类型肌球蛋白的浓度。运动约束导致主动机械性能的显著退化(强直收缩和强度的峰值强度)和两个肌肉的可靠质量损失。肌球蛋白IIa、IIb和IId/x仅在比目鱼肌中表达增强。此外，运动约束同样影响快肌和慢肌的功能特性。我们期望进一步的研究将揭示骨骼肌活动退化的分子机制。

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CHANGES OF MECHANICAL PROPERTIES AND MYOSIN PHENOTYPE OF POSTURAL AND LOCOMOTOR MUSCLES AFTER 21-DAY MOTOR CONSTRAINT

Active mechanical properties of postural and locomotor muscles were investigated in Wistar rats after 21-d motor constraint in small cages. Muscles Soleus and EDL of the right leg were tested mechanically ex vivo; RNA obtained from the left legs was used to determine concentrations of various type myosin. Motor constraint caused a significant degradation of active mechanical properties (peak strength of tetanic contraction and intensity) and reliable mass loss in both muscles. Enhanced expression of myosin IIa, IIb and IId/x was revealed in m. soleus only. Also, motor constraint affected functional properties of equally fast and slow muscles. We anticipate that further investigations will shed light on the molecular mechanisms underlying degradation of the skeletal muscles activity.

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来源期刊

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

0.60

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0.00%

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