21 d干浸渍对toll样受体信号通路相关先天免疫基因表达的影响

Q4 Biochemistry, Genetics and Molecular Biology

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Pub Date : 2022-01-01 DOI:10.21687/0233-528x-2022-56-2-11-19

D. D. Vlasova, A. Sadova, V. Galina, N.S. Guermanov, M. Rykova, E. Antropova, O. Kutko, S. Shulguina, K. Orlova, V. A. Shmarov, E. A. Lysenko, S. Ponomarev

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引用次数: 0

摘要

本研究对10名24 ~ 32岁基本健康男性受试者进行了为期21天的无防制浸泡实验，研究了先天免疫基因的表达。为了评估体液免疫功能，研究了toll样受体编码基因、NF-kB、AP-1、Akt、TLR4介导蛋白;细胞凋亡蛋白，以及cle4e、HSPD1、PTGS2。采用苯酚-氯仿萃取法提取外周血单核细胞CD14+总RNA。随后，逆转录总RNA生成cDNA进行实时PCR。结果显示，这些基因的表达发生了实质性的变化，这表明TLRs功能在转录水平上的变化发生在对21 d DI的免疫反应的最初阶段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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EFFECT OF 21-DAY DRY IMMESRION ON EXPRESSION OF INBORN IMMUNITY GENES ASSOCIATED WITH THE TOLL-LIKE RECEPTORS' SIGNALIING PATHWAYS

Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion experiment without countermeasures. To evaluate the humoral immunity function, genes encoding Toll-like receptors, proteins mediating NF-kB, AP-1, Akt, TLR4; apoptosis proteins, and CLEC4E, HSPD1, PTGS2 were selected. Fenol-cloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLRs function on the transcriptional level were at the very start of the immune response to 21-d DI.

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来源期刊

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

0.60

自引率

0.00%

发文量