模拟重力卸载对比目鱼卫星细胞的影响

Q4 Biochemistry, Genetics and Molecular Biology

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Pub Date : 2022-01-01 DOI:10.21687/0233-528x-2022-56-2-20-29

N. Vilchinskaya, S. Rozhkov, M. Komarova, R.B. Dmitrieva, B. Shenkman

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引用次数: 2

摘要

采用大鼠悬尾模拟重力卸载不同时段后，对比目鱼卫星细胞终末分化的标志物进行了分析。体外培养卫星细胞。Western blotting检测mTORc1、phospho-p70S6K、phospho-ACC、phospho-FOXO1和phospho-FOXO3参与细胞增殖和分化的水平。PCR检测Desmin和АМРКа1的表达。结果表明，尾悬7天和14 d后，大鼠比目鱼肌卫星细胞增殖强度和转录因子FOXO1和FOXO3活性均增加。

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EFFECT OF SIMULATED GRAVITATIONAL UNLOADING ON M. SOLEUS SATELLITE CELLS

Markers of the satellite cells terminal differentiation in m. Soleus were analyzed following different periods of gravitational unloading simulated by rats' tail-suspension. Satellite cells were cultivated in vitro. Western blotting was used to determine levels of mTORc1, phospho-p70S6K, phospho-ACC, phospho-FOXO1 and phospho-FOXO3 involved in cells proliferation and differentiation. Desmin and АМРКа1 expression was determined using PCR analysis. It was shown that 7 and 14 days of tail-suspension lead to the increased intensity of satellite cells proliferation and activities of transcription factors FOXO1 and FOXO3 in rat soleus muscle.

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来源期刊

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

0.60

自引率

0.00%

发文量