利用编码16S核糖体RNA的基因序列对俄罗斯麻风分枝杆菌菌株进行分类鉴定

Q4 Medicine

Infektsionnye Bolezni Pub Date : 2022-01-01 DOI:10.20953/1729-9225-2022-3-67-70

D. Verbenko, D. Deryabin, V. Solomka, A. Karamova, O. Obraztsova, A. Kubanov

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引用次数: 0

摘要

我们分析了编码16S核糖体RNA的基因序列，这些基因包含了来自俄罗斯联邦的两名患者的人类DNA和麻风分枝杆菌DNA的混合物。我们发现我们的序列与存放在美国国家生物技术信息中心(NCBI, USA)的参考菌株序列相匹配。分析使用桑格测序，从皮肤活检标本中分离出微生物和人类DNA的混合物。我们假设与全尺寸rrs基因测序相关的困难可以通过使用rrs启动子区域的物种特异性序列来识别麻风分枝杆菌来解决。关键词:麻风，麻风分枝杆菌，rrs, 16S rRNA

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Taxonomic identification of Mycobacterium leprae strains isolated in the Russian Federation by assessing the sequence of the gene encoding 16S ribosomal RNA

We analyzed the sequence of the gene encoding 16S ribosomal RNA in 2 samples containing a mixture of human DNA and Mycobacterium leprae DNA obtained from two patients from the Russian Federation. We found that our sequence matched the sequence of the reference strain deposited at the National Center for Biotechnology Information (NCBI, USA). The analysis was performed using Sanger sequencing with a mixture of microbial and human DNA isolated from skin biopsy specimens. We assume that the difficulties associated with sequencing of the full-size rrs gene can be addressed by using a speciesspecific sequence of the rrs promoter region to identify M. leprae. Key words: leprosy, Mycobacterium leprae, rrs, 16S rRNA

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来源期刊

Infektsionnye Bolezni Medicine-Infectious Diseases

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The journal publishes original research works, reviews of literature, lectures, methodological recommendations, clinical observations. Main topics: problems of etiology, pathogenesis, clinical manifestations of infectious diseases, new techniques and methods of their diagnosis, prevention and treatment; special attention is paid to the problems of antibacterial and antiviral therapy, the use of immunoglobulins and interferons, and also to intensive therapy of critical states. The journal is in the List of leading scientific journals and periodicals of the Supreme Attestation Committee, where the principal results of doctoral dissertations should be published.