Ipomoea batatas抑制剂对褐家蝇肠道蛋白酶的抑制作用

Q3 Agricultural and Biological Sciences

Journal of Crop Protection Pub Date : 2016-09-19 DOI:10.18869/MODARES.JCP.5.3.461

Balaji Panchal, M. S. Kachole

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引用次数: 2

摘要

小叶螟(Chilo partellus)是一种在世界范围内存在的具有破坏性的高粱和玉米害虫。分离纯化了部ellus肠道蛋白酶(CPGPs)，并对其进行了表征。我们分析了块茎植物中潜在的胰蛋白酶和凝乳胰蛋白酶抑制剂(TIs)，以鉴定cpgp的蛋白酶抑制剂(pi)。枇杷多糖完全抑制了蛋白酶活性和生长，降低了褐藻幼虫的体重。从巴塔塔块茎中纯化了一种PI，并鉴定为cpgp的有效拮抗剂。综上所述，我们发现了在抗性转基因高粱和玉米作物中具有重要应用潜力的Ipomoea batatas抑制剂。

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Inhibition of Chilo partellus (Lepidoptera: Pyralidae) gut proteases with Ipomoea batatas inhibitors

Chilo partellus is a devastating sorghum and maize pest that exists worldwide. C. partellus gut proteases (CPGPs) were isolated, purified and characterized. We analyzed potential trypsin and chymotrypsin inhibitors (TIs) from tuber plants to identify the protease inhibitors (PIs) of CPGPs. PIs from Ipomoea batatas completely inhibited protease activity and growth and led to reduced C. partellus insect larvae weights. One PI was purified from I. batatas tubers and identified as a potent antagonist of CPGPs. In conclusion, we identified Ipomoea batatas inhibitors with significant potential applications in developing resistant transgenic sorghum and maize crops.

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来源期刊

Journal of Crop Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： Journal of Crop Protection is one of the TMU Press journals that is published by the responsibility of its Editor-in-Chief and Editorial Board in the determined scopes. Journal of Crop Protection (JCP) is an international peer-reviewed research journal published quarterly for the purpose of advancing the scientific studies. It covers fundamental and applied aspects of plant pathology and entomology in agriculture and natural resources. The journal will consider submissions from all over the world, on research works not being published or submitted for publication as full paper, review article and research note elsewhere. The Papers are published in English with an extra abstract in Farsi language.