巴西cuiab /MT市区犬血、精液和阴道拭子中布鲁氏菌的检测

IF 0.8 4区农林科学 Q3 AGRONOMY

Ciencia Rural Pub Date : 2023-05-12 DOI:10.1590/0103-8478cr20220455

Sara Maria Dantas da Nóbrega Dias, T. P. P. S. D. Cruz, A. H. Kuczmarski, L. Nakazato, V. Dutra, R. Paz

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引用次数: 0

摘要

摘要:布鲁氏菌病是一种影响动物生殖系统的慢性传染性人畜共患病，造成经济和健康损失。本研究通过比较不同生物样本(血液、精液和阴道分泌物)的PCR阳性程度，并将这些结果与繁殖指标进行比较，对商业犬舍中的布鲁氏菌进行诊断。因此，我们分析了来自邻近城市cuiab和巴西Várzea Grande/MT的狗舍的狗。记录动物生殖史，采集动物血样(n=35);此外，采集男性精液样本(n=9)和女性阴道拭子(n=24)，对布鲁氏菌病进行聚合酶链反应(PCR)检测。结果表明，阴道拭子PCR是一种有效的布鲁氏菌鉴定方法，对于雄性动物，在检测血液样本时阳性结果较多，可能是因为雄性动物处于感染的初期阶段。

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Brucella spp. detection in blood, semen and vaginal swabs in dogs from the urban area of Cuiabá/MT, Brazil

ABSTRACT: Brucellosis is a chronic contagious infectious zoonosis that affects the reproductive system of animals, causing economic and health losses. This study diagnosed Brucella spp. in commercial kennels, comparing PCR positivity in different biological samples (blood, semen, and vaginal secretion), as well as correlating these findings with reproductive indices. Hence, we analyzed dogs from kennels in the neighboring cities of Cuiabá and Várzea Grande/MT, Brazil. The reproductive histories of the animals were obtained and blood samples were collected from all animals (n=35); in addition, semen samples were collected from males (n=9) and vaginal swabs were collected from females (n=24) to perform polymerase chain reactions (PCR) for brucellosis. The findings indicated that vaginal swab PCR is an effective test to identify Brucella spp. For males, there were more positive results when testing blood samples, possibly because the male animals were at the beginning stage of infection.

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来源期刊

Ciencia Rural AGRONOMY-

CiteScore

1.70

自引率

0.00%

发文量

233

审稿时长

2-4 weeks

期刊介绍： The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.