不同培养基对栀子花花粉粒体外活力的影响

IF 0.7 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pesquisa Agropecuaria Brasileira Pub Date : 2022-01-01 DOI:10.1590/s1678-3921.pab2022.v57.03020

Gilmara da Silva Freire, L. Rocha, C. Machado, A. V. C. Silva, A. Lédo

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引用次数: 0

摘要

摘要本研究的目的是在室温条件下，研究不同培养基下美洲Genipa (Genipa americana)花粉粒的活力。试验设计采用3 × 11因子(培养基×孵育时间)完全随机化设计，共设4个重复。在室温下孵育至288 h，每隔24小时对萌发花粉粒数进行分析。100 mg L-1 H3BO3、80 g L-1蔗糖、1.0 g L-1琼脂的培养基发芽率较高至中等，最适合进行栀子花花粉粒离体活力的研究。

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In vitro viability of genipap pollen grains in different culture media

Abstract The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains.

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来源期刊

Pesquisa Agropecuaria Brasileira 农林科学-农业综合

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Agropecuária Brasileira – PAB – is issued monthly by Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, affiliated to Ministry of Agriculture, Livestock and Food Supply. PAB publishes original scientific-technological articles on Plant Physiology, Plant Pathology, Crop Science, Genetics, Soil Science, Food Technology and Animal Science. Its abbreviated title is Pesq. agropec. bras., and it should be used in bibliographies, footnotes, references and bibliographic strips.