植物复合不育剂通过FAM209调控PICK1在大鼠中的作用

IF 0.5 4区生物学 Q4 ZOOLOGY

Pakistan Journal of Zoology Pub Date : 2023-01-01 DOI:10.17582/journal.pjz/20221017071041

Chundan Shi

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引用次数: 0

摘要

本动物实验旨在探讨FAM209和pick1蛋白在SD大鼠鼠害中的调节作用。将SD大鼠随机分为对照组、低剂量组(植物复合不育剂10 mg/kg)和高剂量组(植物复合不育剂20 mg/kg)。采用苏木精、伊红染色观察睾丸组织病理变化。检测睾丸脏器指数、男性精囊、精子。Western blotting检测FAM209、pick1蛋白在睾丸组织中的表达。与对照组相比，模型组大鼠睾丸组织细胞结构受损，对照组、低剂量组大鼠睾丸脏器指数、雄性精囊、精子密度水平均显著升高(P <0.05)。WB结果证实FAM209的表达(P <0.05)。FAM209和pick1蛋白的下调可能参与了鼠害的发生。

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The Role of Plant Complex Sterility Agent in Rats via FAM209 Regulating PICK1

This animal study aimed to investigate the regulatory effect of FAM209, and PICK1protein in SD rats with Rodent pest. SD rats are randomly divided into control group, low (10 mg/kg of plant complex sterility agent) and high dose (20 mg/kg of plant complex sterility agent) groups. Hematoxylin and eosin staining is used to observe the pathological changes of testis tissue. The testis organ index, male seminal vesicle, and sperm were also detected. The expression of FAM209, and PICK1protein in testis tissue are detected by Western blotting. Compared with the control group, the cell structure in the testis tissue of the model group is damaged, and testis organ index, male seminal vesicle, the sperm density level in the control group, low dose group are significantly increased ( P <0.05). The results of WB confirm the expression of FAM209 ( P <0.05). downregulated of FAM209, and PICK1protein may be involved in the development of rodent pest.

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来源期刊

Pakistan Journal of Zoology 生物-动物学

CiteScore

1.10

自引率

16.70%

发文量

306

审稿时长

4.5 months

期刊介绍： Pakistan Journal of Zoology (Pakistan J. Zool.) publishes original articles in English on all aspects of animal life. Generally these articles will be in, or related to one of the following subject areas: Physiology, Cell Biology, Molecular Biology, Genetics, Bioinformatics, Toxicology, Forensic Science, Developmental Biology, Entomology, Parasitology, Microbiology, Biotechnology, Pathology, Palaeontology. Taxonomy, Environmental Biology, Wildlife, Fisheries, Vertebrate and Invertebrate Morphology. Additionally, the journal considers research on health and clinical studies. Short communications are regularly considered, however, uninvited review articles, first records/reports of known species, case reports/studies and survey reports are not published in Pakistan Journal of Zoology.