肉鸡球虫病的特点及亚临床坏死性肠炎的评价

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES
F. Santiani, R. Silva, C. O. Oliveira Júnior, J. A. Withoeft, T. G. Cristo, L. S. Costa, T. Gaspar, R. Casagrande
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引用次数: 0

摘要

摘要:本研究研究了肉鸡球虫病的特征,并评估了坏死性肠炎(NE)的发生情况,以寻求巴西鸡群中这些疾病之间是否存在关联。对32个禽群的256只鸟进行了评价。肉眼和组织病理学病变分级为球虫病和NE。采用聚合酶链反应(PCR)对7种艾美耳球虫进行了肠道内容物检测,并对产气荚膜梭菌进行了选择性厌氧培养和NetB基因的鉴定。球虫病检出率为93.8%。球虫病肉眼病变为1级(27%);柔嫩伊姆斯特(9.7%)和大伊姆斯特(8.9%)。组织病理学检查:十二指肠1级(38.2%);空肠(21.4%);盲肠(9.3%)和回肠(5%)。PCR检测结果显示,微孢子埃希菌(21.9%)、最大埃希菌(18.8%)和尖角埃希菌(3.1%)阳性。肉眼可见的坏死性肠炎病变分为1级(16%)、2级(23%)和3级(10.9%)。组织病理学未见坏死,仅表现为粘膜及粘膜下层出血,有艾美耳球虫属。未分离到A型产气荚膜梭菌netB+,根据组织病理学和netB基因阴性,说明肉眼可见的病变多见于空肠,并非NE的特征。研究表明,由于球虫病的高发生率,许多提示NE的宏观发现实际上归因于艾美耳球虫繁殖引起的非典型病变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of coccidiosis and evaluation of suggestive cases of subclinical necrotic enteritis in broilers
ABSTRACT: This study performed the characterization of coccidiosis in broilers and evaluated the occurrence of suggestive cases of necrotic enteritis (NE), seeking if there is an association between the diseases in Brazilian flocks. Two hundred and fifty-six birds from 32 flocks were evaluated. Macroscopic and histopathological lesions were graduated for coccidiosis and NE. Intestinal content was investigated by polymerase chain reaction (PCR) for seven species of Eimeria and by selective anaerobic culture for Clostridium perfringens and identification of the NetB gene. Flocks positive for coccidiosis represented 93.8%. Macroscopic lesions of coccidiosis were Grade 1 for E. acervulina (27%); E. tenella (9.7%) and E. maxima (8.9%). Histopathological evaluation showed Grade 1 in duodenum (38.2%); jejunum (21.4%); cecum (9.3%) and ileum (5%). PCR demonstrated positivity for E. tenella (21.9%), E. maxima (18.8%), and E. acervulina (3.1%). Suggestive macroscopic lesions of necrotic enteritis ranged from Grade 1 (16%), 2 (23%) and 3 (10,9%). Histopathology indicated the absence of necrosis, showing only hemorrhage in the mucosa and submucosa, with the presence of Eimeria spp. Clostridium perfringens type A netB+ was not isolated, demonstrating that macroscopic lesions found mostly in the jejunum did not characterize NE, based on histopathology and negativity of the NetB gene. The study suggests that, due to the high occurrence of coccidiosis, many macroscopic findings suggestive of NE are, in fact, attributed to atypical lesions caused by the reproduction of Eimeria spp.
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来源期刊
Pesquisa Veterinaria Brasileira
Pesquisa Veterinaria Brasileira 农林科学-兽医学
CiteScore
1.30
自引率
16.70%
发文量
41
审稿时长
9-18 weeks
期刊介绍: Pesquisa Veterinária Brasileira - Brazilian Journal of Veterinary Research (http://www.pvb.com.br), edited by the Brazilian College of Animal Pathology in partnership with the Brazilian Agricultural Research Organization (Embrapa) and in collaboration with other veterinary scientific associations, publishes original papers on animal diseases and related subjects. Critical review articles should be written in support of original investigation. The editors assume that papers submitted are not being considered for publication in other journals and do not contain material which has already been published. Submitted papers are peer reviewed. The abbreviated title of Pesquisa Veterinária Brasileira is Pesqui. Vet. Bras.
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