巴基斯坦主要辣椒种植区引起细菌性枯萎病的番茄枯萎菌的鉴定

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY
Muhammad Naveed Aslam, T. Mukhtar
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引用次数: 1

摘要

对病原体的准确诊断对疾病的正确管理至关重要。因此,本研究对引起辣椒青枯病的Ralstonia solanacearum进行了鉴定,以确定该细菌在不同气候条件和土壤因子的8个农业生态区的生物多样性分布。114株葡萄球菌中,77%呈粘液样生长,23%呈非粘液样生长。同样,黏液样生长的分离株的超敏反应(HR)呈阳性,而非黏液样生长的分离株的HR为阴性。37°C条件下,所有分离株均生长良好,41°C条件下,所有分离株均未产生菌落。114株番茄青霉在所有生化试验中均表现出阳性反应。114株植物分离株中,ⅲ型生物变种占81%,ⅳ型生物变种占19%,ⅲ型生物变种在旁遮普省和信德省分别占37%和70%,ⅳ型生物变种占19%和30%。另一方面,在开伯尔-普赫图赫瓦省和俾路支省,只有生物变种III被记录。同样,在全国4个省的8个农业生态区均观察到生物变种III型,并发现其优势。另一方面,在旁遮普省和信德省的四个农业生态区记录了生物多样性IV。所有分离物均产生750-bp的条带,与茄青霉相对应。结论是III型生物品种在该国广泛流行,需要采取严格的控制措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Ralstonia solanacearum causing bacterial wilt from major chili growing areas of Pakistan
: For proper disease management, accurate diagnosis of the pathogen is essential. Therefore, in the present study Ralstonia solanacearum causing bacterial wilt of chili was characterized to determine the distribution of biovars of the bacterium in the eight agroecological zones with varying climatic conditions and edaphic factors. Among all the 114 isolates of R. solanacearum , 77% showed mucoid growth while 23% isolates gave non-mucoid growth. Similarly, the isolates with mucoid growth were found positive for hypersensitivity response (HR), while those with non-mucoid growth showed negative HR. All the isolates grew well at 37 °C, while none of the isolates produced its colony at 41 °C. All the 114 isolates of R. solanacearum showed positive responses for all the biochemical tests used for confirmation of the bacterium. Out of 114 R. solanacearum isolates, 81% were identified as Biovar III while the remaining 19% were recognized as Biovar IV. Biovar III constituted 37 and 70% in the provinces of Punjab and Sindh, respectively, while Biovar IV formed 19 and 30%. On the other hand, in Khyber Pakhtunkhwa and Balochistan, only Biovar III was recorded. Similarly, Biovar III was observed from all the eight agroecological zones of the four provinces of the country and found to be predominant. On the other hand, Biovar IV was recorded from four agroecological zones located in the provinces of Punjab and Sindh. All the isolates yielded a 750-bp band that corresponded to R. solanacearum . It is concluded that Biovar III is widely prevalent in the country warranting stringent control measures.
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来源期刊
Bragantia
Bragantia AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
2.40
自引率
8.30%
发文量
33
审稿时长
4 weeks
期刊介绍: Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).
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