多诺瓦利什曼原虫原鞭毛菌-80℃低温保存

E. Kaneva
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引用次数: 0

摘要

内脏利什曼病(VL)是由多诺瓦利什曼原虫复合体的专性细胞内原生动物寄生虫引起的重要寄生虫病之一。这种疾病与严重的发病率有关,如果不及时治疗是致命的。人类VL在保加利亚很少见,但该国每年都有当地病例登记。该病的诊断基于临床和流行病学资料以及不同的实验室病理学诊断方法。通过培养方法分离病原体,可以做出极其准确的诊断,但需要时间和材料成本。对分离的寄生虫进行超低温保存,可使其保存较长时间,并可用于后续的免疫学和分子分析。所描述和优化的实验室条件下利什曼原虫原鞭毛虫冷冻保存方法简单可行、可靠,可用于长期保存利什曼原虫原鞭毛虫,且不改变其生存能力和生物学特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CRYOPRESERVATION OF LEISHMANIA DONOVANI PROMASTIGOTES AT -80°C
Visceral leishmaniasis (VL) is one of the important parasitic diseases caused by the obligate intracellular protozoan parasite of Leishmania donovani complex. The disease is associated with severe morbidity and is fatal if left untreated. Human VL is rare in Bulgaria, but local cases are registered annually in the country. Diagnosis of the disease is based on clinical and epidemiological data and the different laboratory methods for ethiological diagnosis. Isolation of the causative agent through cultural methods, allows to make an extremely accurate diagnosis, but require time and cost of materials. Cryopreservation of isolated parasites allows their keeping for a long time and recovery in need of subsequent immunological and molecular analyzes. The described and optimized cryopreservation method of Leishmania species promastigotes in laboratory conditions is easily feasible and reliable and can be used for long-term storage of these parasites without changing their viability and biological characteristics.
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