2016/2017年动物流行病期间保加利亚hpai h5n8检测诊断程序的经验

G. Goujgoulova, G. Stoimenov
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引用次数: 0

摘要

在2016/2017年冬季(10月至1月)的4个月期间,高致病性H5N8是整个欧洲国家的主要血清型。保加利亚是候鸟通过非洲两条主要迁徙航线穿越欧洲的重要地理位置,它受到高致病性H5N8疫情的严重影响。保加利亚于2016年12月19日在维丁地区首次发现高致病性病毒血清型H5N8。随后,在15个不同的行政区域报告了许多野鸟和家禽疫情。截至2017年1月底,在家禽、猎禽、野生水禽和动物园鸟类中检测到高致病性H5N8毒株。观察和报告的症状为身体协调不协调、呼吸困难、共济失调、斜拉肌、水样腹泻、猝死、高死亡率、虚弱和仰卧。在野生鸟类中,只有一些物种的高死亡率数据,主要观察到脑膜充血和脑充血,特别是在达尔马提亚鹈鹕中。这些观察结果显示了研究实验室快速准确地检测和分型这些高致病性病毒的重要性。为了解决这一问题,索非亚甲型流感和纽卡斯尔病国家参考实验室采用了一种改进的实时RT-PCR方法来检测家禽和野生鸟类的N8亚型。本研究的目的是介绍保加利亚在2016/2017年动物流行病期间诊断高致病性H5N8亚型的经验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DIAGNOSTIC PROCEDURE FOR DETECTION OF HPAI H5N8-BULGARIAN EXPERIENCE DURING THE EPIZOOTIC WAVE IN 2016/2017
In the 4 months spanning the 2016/2017 winter (October – January), HPAI H5N8 was the predominant serotype throughout European countries. Bulgaria, an important geographical location for migratory birds crossing Europe through the two major migratory flyways for Africa, was heavily affected with HPAI H5N8 outbreaks. The first detection of HPAI virus serotype H5N8 in Bulgaria was on the 19th of December 2016 in the Vidin region. Subsequently many outbreaks in wild birds and domestic poultry were reported in 15 different administrative regions. By the end of January 2017, the HPAI H5N8 strain was detected in domestic poultry, game birds, wild waterfowl, and zoo birds. The observed and reported symptoms were discoordination, laboured breathing, ataxia, opisthotonos, watery diarrhoea, sudden death, high mortality, weakness, and recumbency. In wild birds data for high mortality was only available for some species, with well-defined hyperaemia of the meninges and brain congestion with a singular haemorrhage being mainly observed, particularly in Dalmatian pelicans. These observations showcase the importance of rapid and accurate detection and subtyping of these HPAI viruses by research laboratories. To tackle this issue, the National Reference laboratory for Influenza A and Newcastle disease in Sofia adapted a modified real-time RT-PCR assay for detection of the N8 subtype in domestic and wild birds. The aim of this study is to present a Bulgarian experience in diagnosis of HPAI H5N8 subtype during 2016/2017 epizootic wave.
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