毛茛属植物氨基酸的鉴定与研究

IF 0.7 Q4 CHEMISTRY, ANALYTICAL
S. Kovalev, A. Golovach, V. Kovalev
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引用次数: 0

摘要

采用荧光和紫外可见反相液相色谱法,采用氟甲基氯甲酸酯自动柱前衍生法测定了灯盏花提取物中的氨基酸含量。这一目标是通过使用邻苯二醛(OPA)对初级氨基酸和9-氟酰氯甲酸甲酯(FMOC)对次级氨基酸的自动衍生化来实现的。然后衍生化与高效液相色谱(HPLC)相结合。应用程序速度快,结果容易重现。对灯盏花中氨基酸组成的认识不足是研究的基础。本文报道了植物原料中16种游离和结合氨基酸(391.41 μg/mg)的含量及不同提取方式对氨基酸谱的影响评价。游离氨基酸总含量为4.66 μg/mg;脯氨酸含量最高,为2.498 μg/mg。结合氨基酸总含量为386.7 μg/mg;优势氨基酸为脯氨酸(146.8 μg/mg)、精氨酸(67.8 μg/mg)、苯丙氨酸(25.8 μg/mg)、天冬酰胺(24.3 μg/mg)、组氨酸(20.4 μg/mg)、丙氨酸(18.2 μg/mg)、丝氨酸(16.6 μg/mg)、缬氨酸(16.0 μg/mg)。9种氨基酸被归为必需氨基酸。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An Identification and Study of Amino Acids of Erigeron annuus Herb
Amino acids in the extract of Erigeron annuus herb were determined using an automatic precolumn derivatization with fluorenylmethyl-chloroformate and reversed-phase liquid chromatography with fluorescence and UV Vis detection. This objective is reached with automatic derivatization using o-phthalaldehyde (OPA) for primary amino acids and 9-Fluorenylmethyl chloroformate (FMOC) for secondary amino acids. Then derivatization integrates into high performance liquid chromatography (HPLC). The applied procedure is fast with easily reproduced results. The insufficient knowledge about amino acids composition of herb of Erigeron annuus is the basis for study. This work reports on content of 16 free and bound amino acids (391.41 μg/mg) in the plant raw material and influence’s evaluation of different extraction types on the amino acid profile. The total content of free amino acids was 4.66 μg/mg; proline prevailed (2.498 μg/mg). The total content of bound amino acids was 386.7 μg/mg; proline (146.8 μg/mg), arginine (67.8 μg/mg), phenylalanine (25.8 μg/mg), asparagine (24.3 μg/mg), histidine (20.4 μg/mg), alanine (18.2 μg/mg), serine (16.6 μg/mg), valine (16.0 μg/mg) were the dominant amino acids. Nine amino acids were classified as essential.
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来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
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