西茜草茎部提取物抗尖孢镰刀菌活性成分研究

IF 0.8 Q3 Agricultural and Biological Sciences

Planta Daninha Pub Date : 2020-01-01 DOI:10.1590/s0100-83582020380100008

R. Akhtar, A. Javaid, M. Qureshi

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引用次数: 9

摘要

摘要:本试验旨在研究茜草芽提取物对尖孢镰刀菌(Fusarium oxysporum f. sp. cepae, FOC)的抑菌作用。在初步的生物测定中，对不同浓度(1 - 5%)的叶、茎和果实提取物进行了FOC测定。所有提取物对病原菌均有较好的抑菌效果。其中，叶提取物对FOC生物量的降低效果最好，达到25-41%。采用正己烷和氯仿两种有机溶剂对甲醇叶提取物进行分馏。以不同浓度(1.56 ~ 200 mg mL-1)对FOC进行了抑菌试验。正己烷和氯仿组分对病原菌具有抑制作用，分别使FOC生物量减少77-93%和80-96%。GC-MS分析表明，正己烷中有24个化合物，氯仿中有4个化合物。在正己烷馏分中，β-谷甾醇含量最高(18.64%)，其次是山梨酸-二(叔丁基二甲基硅基)-叔丁基二甲基硅基酯(12.18%)、10-十八烯酸甲酯(7.90%)和1,2-苯二甲酸二异辛基酯(6.05%)。氯仿馏分中鉴定出的主要化合物为1,3-苯二甲酸、双(2-乙基己基)酯(50.82%)和邻苯二甲酸二辛酯(33.00%)。本研究表明，鸢尾叶甲醇提取物的正己烷和氯仿组分含有有效的抗真菌成分，对FOC具有治理作用。

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Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

ABSTRACT: The present study was carried out to check the antifungal potential of Sisymbrium irio L. shoot extract against Fusarium oxysporum f. sp. cepae (FOC). In preliminary bioassays, different concentrations (1 to 5%) of leaf, stem and fruit extracts were evaluated against FOC. All the extracts were effective against the pathogen. However, the leaf extract was found the most effective causing 25-41% decrease in FOC biomass. The fractionation of methanolic leaf extract was done by two organic solvents namely n-hexane and chloroform. Different concentrations (1.56 to 200 mg mL-1) of these fractions were tested against FOC. The n-hexane and chloroform fractions showed inhibitory activity against the pathogen and resulted in 77-93% and 80-96% reduction in biomass of FOC, respectively. GC-MS analysis showed the presence of 24 compounds in n-hexane and 4 compounds in chloroform fraction. In n-hexane fraction, β-sitosterol (18.64%) was the most abundant compound followed by orotic acid, bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester (12.18%), 10-octadecenoic acid, methyl ester (7.90%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (6.05%). Major compounds identified in chloroform fraction were 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (50.82%) and di-n-octyl phthalate (33.00%). This study concludes that n-hexane and chloroform fractions of methanolic leaf extract of S. irio contain potent antifungal constituents for the management of FOC.

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来源期刊

Planta Daninha Agricultural and Biological Sciences-Plant Science

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Planta Daninha is a scientific journal published by the Brazilian Society of Weed Science (SBCPD - Sociedade Brasileira da Ciência das Plantas Daninhas). Papers submitted for publication must be sent through an electronic system, on http://www.scielo.br/pd. Works may be written in Portuguese, English, or Spanish, and will be accepted after being reviewed and approved by the Editorial Board. Only papers that have not been published or submitted for publication in other media will be accepted. Articles in Portuguese will be translated to English after being properly corrected and authorized by the authors. Planta Daninha has with goal to publish genuine technical-scientific papers and literature reviews from a critical perspective on Biology, weed management, and related topics.