M. Halama, R. Ważny, Matylda Czosnykowska-Łukacka, T. Dobosz
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Tricholoma ustaloides (Agaricales, Basidiomycota) in Poland
. The current distribution of Tricholoma ustaloides Romagn. in Poland is presented, based on literature data and the results of mycological investigations in oak-hornbeam forest associations in the Kotlina Raciborska basin. The paper reports the morphology of the discovered basidiomata, the habitat where they were found, and ITS sequence data for the specimens, and briefly discusses similar species and the ecology of the presented fungus. The location given in the paper is currently the only known station of T. ustaloides in Poland. The importance of secondary habitats for survival of this taxon is stressed. The final extension was carried out at 72°C for 5 min. The PCR products were visualized by agarose electrophoresis. The ITS rDNA amplicons were sequenced with ITS4 primer by the Lab-oratory of Molecular Biology of Adam Mickiewicz Uni versity in Poznań (Poland). All obtained sequences were analyzed by Chromas (www.technelysium.com.au) and compared with the sequences deposited in the UNITE and NCBI databases using the BLASTn algorithm.
期刊介绍:
Polish Botanical Journal is an international journal publishing original papers covering various aspects of vegetation science, plant and fungi biodiversity, biosystematics (including plant anatomy, cytology and embryology), phytogeography, evolution and ecology. All papers are considered by at least two reviewers. Polish Botanical Journal is issued twice per year. The two issues form one annual volume.