利用CRISPR/Cas9技术选育烟草LJ911抗性PVY

IF 1.5 4区农林科学 Q2 Agricultural and Biological Sciences

Crop Breeding and Applied Biotechnology Pub Date : 2021-01-01 DOI:10.1590/1984-70332021V21N1A6

Ren Ru-yi, Zheng-Liu Qiang, Ni Futai, J. Qiu, Xiuqing Wan, Jicheng Wei

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引用次数: 7

摘要

马铃薯Y病毒(PVY)是烟草的主要病害之一。最近的研究表明，Va基因(Ntab0942120)决定了烟草对PVY的易感性，Va基因产物与PVY基因组连接蛋白(VPg)相互作用，启动PVY基因组翻译过程，最终导致烟草被病毒全身感染。本研究以烟草品种LJ911为受体材料进行基因编辑。通过CRISPR/Cas9技术敲除Va基因，建立T1代无转基因纯合子编辑植株。病理试验表明，经过编辑的植株获得了PVY抗性。因此，在本研究中产生的编辑材料代表了潜在的有用的遗传资源，为培育抗pvy烟草。

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Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology

Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum). Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVYresistant tobacco.

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来源期刊

Crop Breeding and Applied Biotechnology 农林科学-农艺学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.