用杂环二硫化物分析同型半胱氨酸和含硫醇的化合物

Q4 Chemistry
A. Shcherbatykh, O. I. Askalepova, I. A. Estrin
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引用次数: 0

摘要

生物低分子氨基硫醇,如同型半胱氨酸,在紫外可见范围内具有较弱的吸收特性。因此,氨基硫醇分析需要使用硫醇专用化学传感器或衍生化程序。杂环二硫化物是一类衍生化剂。然而,在分析实践中广泛使用的二硫化物,如5,5'-二硫比斯-(2-硝基苯甲酸),有几个缺点:可接受的pH范围有限,灵敏度低,易水解。因此,寻找有效的衍生试剂仍在继续。本研究采用2,2′-二硫代比斯[苯并恶唑]和8,8′-二硫代比斯-喹啉二硫化物分析同型半胱氨酸。建立了精确灵敏的同型半胱氨酸检测方法,包括分光光度法、毛细管电泳(CE)、高效液相色谱(HPLC)和动力学法,检测限为1.1·10-6 - 7.8·10-6 M,相对标准偏差为1.5-4.6%。所提出的二硫化物也已成功地用于分析人类尿液样品中的巯基含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of homocysteine and thiol-containing compounds using the heterocyclic disulfides
Biological low-molecular aminothiols, such as homocysteine, are characterized by the weak absorption in the UV – visible range. Therefore, aminothiols analysis requires using thiol-specific chemosensors or derivatization procedure. Heterocyclic disulfides belong to a large group of derivatizing agents. However, the disulfides widely used in the analytical practice, such as 5,5'-dithiobis-(2-nitrobenzoic acid), have several disadvantages: limited acceptable pH range, low sensitivity, susceptibility to hydrolisys. Hence, the search for the effective derivatizing agents continues. In the current work, 2,2'-dithiobis[benzoxazole] and 8,8'-dithiobis-quinoline disulfides were used for the analysis of homocysteine. Precise and sensitive methods (homocysteine detection limit in the range of 1.1·10-6–7.8·10-6 M, relative standard deviation in the range of 1.5–4.6%) for the homocysteine determination have been developed, including spectrophotometric method, capillary electrophoresis (CE), high-performance liquid chromatography (HPLC) and kinetic method with the spectrophotometric detection. The proposed disulfides have also been successfully used in the analysis of sulfhydryl groups content in the sample of human urine.
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来源期刊
Analitika i Kontrol
Analitika i Kontrol Chemistry-Analytical Chemistry
CiteScore
0.90
自引率
0.00%
发文量
15
期刊介绍: Analitika i Kontrol is a scientific journal covering theoretical and applied aspects of analytical chemistry and analytical control, published since autumn 1997. Founder and publisher of the journal is the Ural Federal University named after the first President of Russia Boris Yeltsin (UrFU, Ekaterinburg).
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