二甲基乙酰胺和二甲基亚砜结合葡萄糖和蛋黄冷冻保存corruscans伪鸭嘴兽精液

IF 2 4区生物学 Q1 ZOOLOGY

Neotropical Ichthyology Pub Date : 2023-02-20 DOI:10.1590/1982-0224-2022-0071

Johanna Maritza Cano Velarde, N. M. Bastos, Laícia Carneiro-Leite, Laís Pedroso Borges, E. G. Vieira, R. Veríssimo‐Silveira, A. Ninhaus‐Silveira

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引用次数: 0

摘要

摘要:本研究旨在建立corruscans伪platystoma精液的冷冻保存方案。为此，用单剂量鲤鱼垂体提取物(5 mg/kg体重)诱导成熟雄鱼。收集并评估精液。用两种低温保护剂组成稀释剂:二甲乙酰胺(DMA)和二甲亚砜(Me2SO)，以两种浓度(8%和10%)，+ 5.0%葡萄糖+ 10%蛋黄。将精液按1:4的比例稀释(精液:填充剂)，包装在0.5 mL吸管中，在液氮蒸汽中冷冻在干燥的托运人容器中。解冻后对精子动力学、精子形态和DNA完整性进行评价。雄斑拟platystoma corruscans产生的精液精子活力为80%。解冻后，各处理提供的精液总精子活力bbb40 %，两者之间无显著差异(P < 0.05)，其他精子动力学参数也无显著差异(P < 0.05)。DMA处理提供了较小的配子DNA片段。在新鲜和冷冻保存的精液中都发现了精子畸形，在解冻的corruscans精液样本中发现的精子畸形略有增加。

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Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen

Abstract This study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to compose the diluents: dimethyl acetamide (DMA) and dimethyl sulfoxide (Me2SO), in two concentrations (8% and 10%), + 5.0% glucose + 10% egg yolk. The semen was diluted in a 1: 4 ratio (semen: extender), packed in 0.5 mL straws and frozen in a dry shipper container in liquid nitrogen vapors. After thawing, sperm kinetics, sperm morphology and DNA integrity of cryopreserved sperm were evaluated. Pseudoplatystoma corruscans males produced semen with sperm motility > 80%. After thawing, all treatments provided semen with total sperm motility > 40%, with no significant difference (P < 0.05) between them, as well as between the other sperm kinetic parameters evaluated. The treatments with DMA provided a smaller fragmentation of the DNA of the gametes. Sperm malformations were identified in both fresh and cryopreserved semen, with a slight increase in these malformations being identified in sperm from thawed P. corruscans semen samples.

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来源期刊

Neotropical Ichthyology 生物-动物学

CiteScore

2.80

自引率

17.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Neotropical Ichthyology is the official journal of the Sociedade Brasileira de Ictiologia (SBI). It is an international peer-reviewed Open Access periodical that publishes original articles and reviews exclusively on Neotropical freshwater and marine fishes and constitutes an International Forum to disclose and discuss results of original research on the diversity of marine, estuarine and freshwater Neotropical fishes. -Frequency: Four issues per year published only online since 2020, using the ‘rolling pass’ system, which posts articles online immediately as soon as they are ready for publication. A searchable and citable Digital Object Identifier (DOI) is assigned to each article immediately after online publication, with no need to await the issue’s closing. -Areas of interest: Biology, Biochemistry and Physiology, Ecology, Ethology, Genetics and Molecular Biology, Systematics. -Peer review process: The Editor-in-Chief screens each manuscript submitted to Neotropical Ichthyology to verify whether it is within the journal’s scope and policy, presents original research and follows the journal’s guidelines. After passing through the initial screening, articles are assigned to a Section Editor, who then assigns an Associate Editor to start the single blind review process.