葡萄糖转运蛋白2 (GLUT2)基因在脾拟虾(拟虾目:拟虾科)胚胎、幼鱼和成鱼中的表达及特性研究

IF 2 4区生物学 Q1 ZOOLOGY

Neotropical Ichthyology Pub Date : 2022-01-01 DOI:10.1590/1982-0224-2021-0171

A. Castillo-Collado, C. Frías‐Quintana, V. Morales-García, C. Alvarez-Villagomez, G. Asencio-Alcudia, E. Peña‐Marín, Gil Martínez-Bautista, L. D. Jiménez‐Martínez, C. Álvarez‐González

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引用次数: 0

摘要

摘要:墨西哥海湾斯努克(Petenia splendida)是墨西哥东南部一种极具经济价值的肉食性慈鲷。尽管该物种在商业养殖方面具有良好的潜力，但有关其营养、生理和生殖代谢途径的信息却很少。目前的研究重点是葡萄糖转运蛋白2 (glut2)在孵化后5、10、15、20、25和30天的胚胎和幼虫以及成鱼的肝脏、肠道、肾脏、肌肉、心脏、睾丸、鳃、胃、胰腺和大脑中的表达。获得glut2的部分序列，设计特异性qPCR引物。在5、15和20 dph时，胚胎的表达量低于幼虫。20 dph时幼虫表达量最高，25和30 dph时表达量最低。在成人中，最高表达水平出现在肝脏和肠道。我们的研究结果表明，谷氨酸2在成虫各组织中的表达存在差异，并且在幼虫发育过程中存在波动。

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Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae)

Abstract Bay snook (Petenia splendida) is a carnivorous cichlid species with excellent economic value in Southeast Mexico. Although this species presents an excellent potential for commercial aquaculture, the information about its nutritional, physiological, and reproductive metabolic pathways is meager. The current study focuses on the expression of glucose transporter 2 (glut2) in embryos and larvae at 5, 10, 15-, 20-, 25-, and 30-days post-hatch (dph) and in the liver, intestine, kidney, muscle, heart, testicle, gill, stomach, pancreas, and brain of adult fish. The partial sequence of glut2 was obtained, and specific qPCR primers were designed. In embryos, the expression was lower compared to larvae at 5, 15, and 20 dph. The highest expression in larvae occurred at 20 dph and the lowest at 25 and 30 dph. Maximum expression levels in adults occurred in the liver and intestine. Our results show that glut2 is expressed differentially across tissues of adult bay snook, and it fluctuates during larval development.

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来源期刊

Neotropical Ichthyology 生物-动物学

CiteScore

2.80

自引率

17.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Neotropical Ichthyology is the official journal of the Sociedade Brasileira de Ictiologia (SBI). It is an international peer-reviewed Open Access periodical that publishes original articles and reviews exclusively on Neotropical freshwater and marine fishes and constitutes an International Forum to disclose and discuss results of original research on the diversity of marine, estuarine and freshwater Neotropical fishes. -Frequency: Four issues per year published only online since 2020, using the ‘rolling pass’ system, which posts articles online immediately as soon as they are ready for publication. A searchable and citable Digital Object Identifier (DOI) is assigned to each article immediately after online publication, with no need to await the issue’s closing. -Areas of interest: Biology, Biochemistry and Physiology, Ecology, Ethology, Genetics and Molecular Biology, Systematics. -Peer review process: The Editor-in-Chief screens each manuscript submitted to Neotropical Ichthyology to verify whether it is within the journal’s scope and policy, presents original research and follows the journal’s guidelines. After passing through the initial screening, articles are assigned to a Section Editor, who then assigns an Associate Editor to start the single blind review process.