Electromagnetic and acoustic technologies in antibacterial preparation development

Subject and Purpose. The present paper is concerned with the use of wave technologies in the development of antibiotics-alternative approaches for pathogenic microflora suppression. Lactobacilli strains picked in different ecological niches and their activity against pathogenic strains are studied with a focus on a targeted modification of adhesive and antagonistic properties of lactobacilli by exposing them to low-intensity electromagnetic (EM) fields and the ultrasound. Methods and Methodology. Lactobacilli picked in different ecological niches are experimentally studied, including (1) standard strains from probiotic preparations and (2) circulating strains picked in humans and bees. For the ultrasonic and electromagnetic radiation sources, G3-109 and G3-F and G4-141 and G4-142 generators are taken, respectively. The adhesive properties of Lactobacillus spp. strains and their antagonistic activity are estimated against C. diphtheriae, S. aureus and yeast-like fungi of Candida genus in aerobic and microaerophilic culture conditions. Statistical technology is employed in the data processing and analysis. Results. It has been established that L. plantarum strains picked in the gut of healthy bees are most antagonistic towards pathogens. It has been demonstrated that the priority culture conditions for lactobacilli are microaerophilic conditions simulating their stay in vivo. It has been shown that it is possible to modify properties of microorganisms by their exposure to ultrasound and low-intensity electromagnetic fields in narrow bands of the EHF range. The effect efficiency versus frequency has a dispersion character. Individual features of various pathogenic strains have been recognized. Conclusion. The obtained results open up prospects for electromagnetic and acoustic technologies in the development of safe alternative means to antagonize persisting pathogens and increase human body resilience.