高粱蛋白构象的13C - ss-NMR奇异值分解与拟合

IF 1 4区化学 Q4 POLYMER SCIENCE

Polimeros-ciencia E Tecnologia Pub Date : 2022-01-01 DOI:10.1590/0104-1428.20210082

T. Ribeiro, J. A. Scramin, J. Rodrigues, Rubens Bernardes Filho, L. Colnago, L. A. Forato

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引用次数: 0

摘要

摘要甜高粱BR501籽粒中的不水溶性蛋白Kafirins由于其疏水特性，已成为制备可食用食品涂层的一种替代方法。本文采用13c固体核磁共振光谱法，利用羰基峰面积测定了还原卡菲蛋白(SSr)和未还原卡菲蛋白(SSu)的二级结构(SS)含量。通过洛伦兹函数拟合信号，SSr和SSu的α-螺旋结构分别为56%和59%，β-片结构分别为40%和12%。用奇异值分解- SVD方法鉴定，SSr和SSu的α-螺旋结构分别为55%和49%，β-片结构分别为12%和8%。由于SVD不依赖于算子，且α-螺旋(0.96%)和β-薄片(0.91%)的相关系数较高，是一种可靠的不溶性蛋白SS定量方法

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13C ss-NMR Singular value decomposition and fitting for sorghum proteins conformation elucidation

Carlos, SP, Brasil *tatianaribeiro@ufscar.br Obstract Kafirins, water-insoluble proteins from Sweet Sorghum BR501 grains, have been an alternative to prepare edible coatings for food due to their hydrophobic character. In this work, the secondary structures (SS) content of reduced (SSr) and unreduced (SSu) kafirins were determined by 13 C solid-state-NMR spectroscopy using areas of carbonyl peak. The SS elucidate by fitting the signal with the Lorentzian function shows 56% and 59% of α-helix and 40% and 12% of β-sheet structures for SSr and SSu, respectively. The SS also were elucidated by a Singular value decomposition- SVD method shows 55% and 49% of α-helix and 12% and 8% of β-sheet structure for SSr and SSu, respectively. Since SVD does not depend on the operator and has higher correlation coefficients for α-helix (0.96%) and β-sheet (0.91%), it is a reliable method to quantify the SS of insoluble proteins using

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来源期刊

Polimeros-ciencia E Tecnologia 化学-高分子科学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Polímeros is a quarterly publication of the Associação Brasileira de Polímeros - ABPol (Brazilian Polymer Association), which publishes Review Articles, Original Articles and Short Communications, disclosing advances in the knowledge of Polymer Science and Technology.