mutR基因在emm12型链球菌菌株的新陈代谢和毒性方面的作用

IF 0.2 Q4 INFECTIOUS DISEASES

Infektsiya i Immunitet Pub Date : 2015-02-03 DOI:10.15789/2220-7619-2014-4-339-346

A. A. Zutkis, B. Milman, A. Dmitriev

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引用次数: 0

摘要

本研究研究了化脓性链球菌(Streptococcus pyogenes, emm12)的mutR调控蛋白基因的功能作用。该菌株的mutR基因失活。97和没有。152通过插入突变。发现mutR基因失活会影响细菌在液体培养基中的生长动态和特性。与野生型菌株相比，突变菌株分泌核酸酶的表达显著降低。双向电泳和质谱分析显示突变菌株与野生型菌株在蛋白质表达数量上存在差异。mutR基因的失活对化脓性葡萄球菌粘附人上皮细胞的能力产生负面影响。最后，对菌株的毒力特性进行了研究。152株[mutR]突变株与no. 1突变株相比减少了4.7倍。第152名，而第15名。97[mutR]突变株与no.突变株相比无毒性。97由于插入性失活的mutR基因。

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РОЛЬ ГЕНА mutR В МЕТАБОЛИЗМЕ И ВИРУЛЕНТНОСТИ ШТАММОВ STREPTOCOCCUS PYOGENES ГЕНОТИПА emm12

In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.

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来源期刊

Infektsiya i Immunitet INFECTIOUS DISEASES-

CiteScore

1.40

自引率

50.00%

发文量

审稿时长

10 weeks

期刊介绍： Journal "Infektsiya i immunitet" ("Russian Journal of Infection and Immunity") established by Northwest Branch of RAMS, St. Petersburg Pasteur Institute and the St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists, with the participation of the St. Petersburg branch of All-Russian Practical Society of epidemiologists, microbiologists and parasitologists at St. Petersburg and Leningrad region. The journal is devoted to numerous aspects of the interaction between different microorganisms and the host organism. Journal is of interest for microbiologists, immunologists, epidemiologists and clinicians. The most detailed discussion of the following questions: • molecular basis of infections caused by pathogenic bacteria, fungi and parasites; • mechanisms of pathogenicity of microorganisms; • the impact of microbial virulence factors on host cells; • factors and mechanism to protect the host from infection; • factors of nonspecific and specific immunity; • experimental models of infectious disease; • development of vaccines and nonspecific anti-infectious defense.