原位荧光显微镜下单链DNA分子离子与二甲基二十八烷基氯化铵在异辛烷- 1-辛醇混合物中的相转移动力学研究

IF 0.6 4区工程技术 Q4 CHEMISTRY, MULTIDISCIPLINARY

Solvent Extraction Research and Development, Japan Pub Date : 2021-01-01 DOI:10.15261/SERDJ.28.1

S. Tsukahara, Shohei Inoshita, T. Fujiwara

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引用次数: 0

摘要

在高分辨率荧光显微镜下，研究了N、N -二甲基- N、N -二十八烷基氯化铵对单个巨DNA分子离子的溶剂萃取，并成功地观察了它们从水相到异辛烷- 1-辛醇混合物(油)相的相转移。在水相中提取的dna处于随机线圈状态，在油相中提取的dna处于不完全球状状态，而存在于界面处的dna则几乎处于球状状态。从这些构象变化出发，提出了DNA在界面处的相转移机理。

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In Situ Fluorescence Microscope Measurements of the Phase Transfer Dynamics of Single DNA Molecular Ions with Dimethyldioctadecylammonium Chloride into Isooctane–1-Octanol Mixture

The solvent extraction of single giant DNA molecular ions by N , N -dimethyl- N , N -dioctadecylammonium chloride was investigated under a fluorescence microscope with high resolution and their phase transfers from the water phase to the isooctane–1-octanol mixture (oil) phase were observed successfully. DNAs in the water phase were in the random-coil state, and those extracted in the oil phase were in the imperfect globule state, whereas those existing at the interface were almost in the globule state. From these conformation changes, the phase transfer mechanism of DNA at the interface was proposed.

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来源期刊

Solvent Extraction Research and Development, Japan 工程技术-工程：化工

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

1 months

期刊介绍： Solvent Extraction Research and Development, Japan (Solvent Extr. Res. Dev., Jpn.) is a periodical issued from Japan Association of Solvent Extraction (JASE) containing papers dealing with all aspects of solvent extraction and their related methods, underlying principles, and materials. Original articles, notes, technical reports, and critical reviews will be considered for publication. Original articles must be of reasonably broad scope and significance to the solvent extraction. Notes will originally describe novel work of a limited nature or especially significant work in progress. Authors can contribute some information of novel techniques, equipment or apparatus, reagents and diluents for solvent extraction as technical reports. Critical reviews will be received from the authors to whom the editorial committee asked to contribute.