聚丙烯酰胺等电聚焦电泳技术对肯塔基31号高羊茅的酯酶鉴别

IF 1.7 4区农林科学 Q2 AGRONOMY

Seed Science and Technology Pub Date : 2023-01-01 DOI:10.15258/sst.2023.51.1.02

Yuehua Wu, Laura Berríos-Ortiz, Ernest Allen, Todd Erickson, Elizabeth Stewart

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引用次数: 0

摘要

聚丙烯酰胺等电聚焦电泳(PA-IEF)为肯塔基31号高羊茅品种的鉴定提供了替代或补充检测方法。在两年的时间里，对276株高羊茅样品进行了田间生长试验和PA-IEF凝胶电泳程序的新应用进行了比较。PA-IEF凝胶试验清楚地发现了肯塔基31号品种独特的4波段模式，这在其他21个商业高羊茅品种中没有发现。这种独特的4波段模式可以用来区分肯塔基31和其他高羊茅品种。最后，该方法提供的结果比现场生长试验所需的时间短得多。

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Polyacrylamide isoelectric focusing electrophoresis for differentiating Kentucky 31 tall fescue by esterase enzyme

Polyacrylamide isoelectric focusing electrophoresis (PA-IEF) provides alternative or supplementary testing for identifying the Kentucky 31 variety of tall fescue. Over a two-year period, field grow-out tests and a novel use of PA-IEF gel electrophoresis procedures were compared for 276 tall fescue samples. The PA-IEF gel test clearly identified a unique 4-band pattern in the Kentucky 31 variety, which was not detected in 21 other commercial varieties of tall fescue. This unique 4-band pattern can be used to differentiate between Kentucky 31 and other tall fescue varieties. Finally, the method provided results over a much shorter time frame than field grow-out tests.

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来源期刊

Seed Science and Technology 农林科学-农艺学

CiteScore

3.00

自引率

28.60%

发文量

审稿时长

>36 weeks

期刊介绍： Seed Science and Technology (SST) is an international journal featuring original papers and articles on seed quality and physiology related to seed production, harvest, processing, sampling, storage, genetic conservation, habitat regeneration, distribution and testing. A journal that meets the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality. Published every April, August and December.