生物印迹脂肪酶无溶剂酯化反应动力学研究

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
M. Matsumoto, Y. Matsumoto
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引用次数: 2

摘要

为了避免使用对环境造成负担的化合物,本研究的重点是无溶剂酶促反应。研究了羧酸预处理脂肪酶对丙酸与异戊醇无溶剂酯化反应的催化活性和动力学。研究了羧酸的生物印迹效应对酯化反应收率和速率的提高。我们发现异戊醇对无溶剂酶促酯化反应没有抑制作用,并且在大量过量异戊醇对丙酸的印迹作用很大。从动力学分析来看,与未处理的脂肪酶相比,印迹主要提高了脂肪酶与丙酸之间的催化反应速率常数,而不是亲和力。脂肪酶的生物印迹处理对无溶剂酯化反应的产率和动力学都有很好的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase
To avoid the use of compounds that burden the environment, a solvent-free enzymatic reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl alcohol. The enhancements of the esterification yields and rates by the bio-imprinting effects of carboxylic acids were observed. We found no inhibition of isoamyl alcohol on the solvent-free enzymatic esterification, and obtained a large imprinting effect under a largely excessive amount of isoamyl alcohol to propionic acid. From the kinetic analysis, the imprinting of lipases mainly enhanced the catalytic reaction rate constant rather than the affinity between lipase and propionic acid compared with untreated lipase. The bio-imprinting treatment of lipase is found to be very effective for the yield and kinetics in solvent-free esterification.
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来源期刊
Chemical and Biochemical Engineering Quarterly
Chemical and Biochemical Engineering Quarterly 工程技术-工程:化工
CiteScore
2.70
自引率
6.70%
发文量
23
审稿时长
>12 weeks
期刊介绍: The journal provides an international forum for presentation of original papers, reviews and discussions on the latest developments in chemical and biochemical engineering. The scope of the journal is wide and no limitation except relevance to chemical and biochemical engineering is required. The criteria for the acceptance of papers are originality, quality of work and clarity of style. All papers are subject to reviewing by at least two international experts (blind peer review). The language of the journal is English. Final versions of the manuscripts are subject to metric (SI units and IUPAC recommendations) and English language reviewing. Editor and Editorial board make the final decision about acceptance of a manuscript. Page charges are excluded.
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