抑制SRSF3通过调节PI3K/AKT/mTOR信号通路减轻胃癌细胞的增殖和迁移

IF 0.8 4区生物学 Q4 BIOLOGY

Folia Biologica-Krakow Pub Date : 2021-01-01 DOI:10.14712/fb2021067030102

J. Xie, Y. Sun, Qiaolin Xu

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引用次数: 3

摘要

本研究旨在探讨富丝氨酸/精氨酸剪接因子3 (SRSF3)对胃癌(GC)细胞增殖和迁移的影响。Western blotting检测GC组织和细胞系中SRSF3水平。功能测定法评价GC细胞的增殖、迁移和侵袭能力。Western blotting检测PI3K/AKT/mTOR通路。SRSF3在GC中表达异常，表达水平显著升高。SRSF3敲除显著抑制GC进展。SRSF3敲低显著抑制PI3K/AKT/mTOR信号的激活。抑制SRSF3可减轻GC细胞的增殖和迁移，这一过程是通过PI3K/ AKT/mTOR信号失活介导的。靶向SRSF3可能是对抗GC的有希望的策略。

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Inhibition of SRSF3 Alleviates Proliferation and Migration of Gastric Cancer Cells by Regulating the PI3K/AKT/mTOR Signalling Pathway.

This study was aimed to investigate the impact of serine/arginine-rich splicing factor 3 (SRSF3) on the proliferation and migration of gastric cancer (GC) cells. SRSF3 levels in GC tissues and cell lines were measured by Western blotting. Functional assays were used for evaluation of GC cell proliferation, migration and invasion. The PI3K/AKT/mTOR pathway was then examined by Western blotting. SRSF3 exhibits abnormal expression for the significantly increased levels in GC. SRSF3 knockdown significantly suppressed GC progression. SRSF3 knockdown significantly inhibited activation of PI3K/AKT/mTOR signalling. Inhibition of SRSF3 alleviates proliferation and migration of GC cells, and this process is mediated by inactivation of PI3K/ AKT/mTOR signalling. Targeting SRSF3 may be a promising strategy to combat GC.

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来源期刊

Folia Biologica-Krakow 医学-生物学

CiteScore

1.10

自引率

14.30%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Biologica (Kraków) is an international online open access journal accepting original scientific articles on various aspects of zoology: phylogeny, genetics, chromosomal studies, ecology, biogeography, experimental zoology and ultrastructural studies. The language of publication is English, articles are assembled in four issues per year.